Bioengineering unleashed! Substrate structuration & functionalization = better cell models. Tools for better cell sample preparation for cell biology & cryo-ET.
Paris, France
Joined May 2016
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Alvéole retweeted
Keith Hamilton from the @Alushin_Lab at Rockefeller University gave a deep dive into the first step of any structural biological workflow: sample preparation. Through test cases he showed the balance of what is easily doable versus preserving biology.
alushinlab.rockefeller.edu/p…
Alvéole retweeted
Merry Christmas everyone!
Epithelial cells pattern on @AlveoleLab & imaged on @Abberior at @RosFrankInst by lab members Xuemeng, Dimitrios, Chelsea et al. :) – at Oxford, England
Alvéole retweeted
🎄It’s Beginning to Look a Lot Like Christmas... Under the Microscope!🔬
Why buy Christmas trees when you can "grow" one using micropatterning ( @AlveoleLab ) and @AllenInstitute 's cells with LaminB1 "ornaments"?
Happy #FluorescenceFriday !
Alvéole retweeted
To challenge the limits of the system, we studied conditions with many beads in the microwells. We then looked at the fraction of weak beads moving, as a function of competition strength (defined by the number of strong beads).
Alvéole retweeted
10. We then demonstrated that when there is turnover, weak structures (from beads or micropatterns) can grow when they are in competition with strong networks. Turnover enables the weakest to exist!
Alvéole retweeted
9. Therefore, without turnover, the actin monomer is limiting and the available monomer pool is taken over by dense structures in a “winner-take-it-all” scenario.
Alvéole retweeted
8. To challenge those results in another geometry, the amazing @cguerin38 developed a protocol to generate actin networks with various densities on lipidic micropatterns at the bottom of our microwells (with the Primo @AlveoleLab).
Alvéole retweeted
And a huge thank you to all the sponsors of the 2024 CryoET workshop — @AlveoleLab, @awscloud, @DDNStorage, @GatanMicroscopy, @MiTeGen, @SubangstromLLC and @thermofisher — for helping to make this hands-on workshop possible!
ALT Decorative graphic with logos of the sponsors
Alvéole retweeted
Making some cell adhesion micropatterns for standardizing cell shape. Each crossbow is approximately the size of a mammalian cell. #primo_power @AlveoleLab
Alvéole retweeted
With a host of #lightmicroscopy solutions from leading suppliers we are bound to have a system that will benefit yor research.
Come and speak to us at #LMA2024
@ONIbio @NanoLiveLtd @phioptics @AlveoleLab @Hirox @Etaluma
Get your free AXT Foldscope 👉 bit.ly/3wOkW62
Alvéole retweeted
Are you looking for a better way to analyse #organoids #spheroids and other cellular structures?
@Alveole Primo allows you to engineer custom microenvironments to create more reliable and reproducible cell models.
Talk to us at #LMA2024 or www2.axt.com.au/primo
@LightMicroAus
Alvéole retweeted
@Cairn_GRog is in Philadelphia attending #BPS2024 with the @BiophysicalSoc. He is at booth #619 with @ChromaVT - in the photo is our #openFrame #modular #microscope with @CrestOptics #Cicero and @AlveoleLab #primo connected. If there, please go and say hi!
Alvéole retweeted
Does your research involved #proteins?
Come visit our stand at #LorneProteins and let us know what you are wanting to know about characterising your proteins.
#XRD #mircoED #proteomics #superresolution #microscopy #platehotels @Rigaku @ONIhq @JANSi @Alveolelab #micropatterning
Alvéole retweeted
Great news for cellular imaging enthusiasts! 🔬 Now, you can study ultrastructural details within the cell's native environment at sub-nanometer resolution! How? ⤵️
bio-protocol.org/en/bpdetail…
#cellular #imaging #microscopy #fluorescence #cryoem
(🧵👇)
Alvéole retweeted
New equipment @AlveoleLab installed this week! Full of new ideas. Stay tuned… and more to come next week
Alvéole retweeted
Just some breast epithelial cells scaring this Halloween season! (and hopefully not scaring good experiments 🙏)
#PRIMO #micropatterning @AlveoleLab @IBECBarcelona
Alvéole retweeted
With the PRIMO device from @AlveoleLab, we have also developed a new technique for generating micropatterns of controlled geometry, with high-friction zones juxtaposed to low-friction zones. In this case, the contraction was biased toward the region with higher friction.
Alvéole retweeted
Pleased to present our latest work, where we combine engineered filament capping complexes with @AlveoleLab PRIMO micro-patterning of myosin motors to create a system for studying force-regulated actin binding interactions in higher-order networks
biorxiv.org/cgi/content/shor…
Alvéole retweeted
We micropatterned lymphocytes on EM grids to facilitate cryo-FIB & cryo-ET, revealing networks of filaments on the Jurkat cell surface. Was awesome to work with @dr_slo on this side project during my postdoc in @Dunn_Lab! #teamtomo @AlveoleLab biorxiv.org/content/10.1101/…
Alvéole retweeted
Last but not least #20ans our co-director Serge Monneret presenting various solutions to transfer from lab to companies or to support these collaborations/codevelopments, and highlight the four different companies out of our labs @abbelight @AlveoleLab @inscoper LightCore
