Cryo-EM core NIEHS. Opinions are my own.
Durham, NC
Joined September 2014
- Tweets286
- Following155
- Followers298
- Likes303
Pinned Tweet
Met amazing people at my first #3DEM @GRC this week!
For those who missed #3DEM, I am pleased to announce that I will be joining @Princeton @PrincetonMolBio in just 2 months as a core manager to lead the cryoEM efforts. Stay tuned for great science to come! #CryoEM #Teamtomo
#CryoEM SmartScope v0.9.2 is out!
Here are a few upgrades:
- Aperture Control w/ SerialEM 4.1-beta @Nexperion
- Great progress on CryoARM support.
- More flexibility about data storage locations.
- Easier installation procedure.
Full notes at: github.com/NIEHS/SmartScope/…
Please check out @scipion_em smartscope plugin to feedback your preprocessing data to SmartScope.
Now in Beta!
github.com/scipion-em/scipio…
I received a broken litre of hot sauce from @Walmart and other items that got ruined during shipping
No offer to reship nor honor the prices at the time of purchase
They offered a refund- that's it
#doneWithWalmart
#CustomerExperience
Wouldn't have happened @Target
From having first-hand experience with this software, it is incredible.
The tomo part particularly, fills a gap in the field. Never build a tomogram manually again.
And for SPA preprocessing, you can't get faster than this with parallelization up to your cluster limits.
Out today from the Bartesaghi lab! nextPYP is a turn-key framework for single-particle cryo-ET that streamlines complex data analysis pipelines, from pre-processing of tilt series to high-resolution refinement, for efficient analysis of large datasets. nature.com/articles/s41592-0…
Jonathan Bouvette retweeted
Out today from the Bartesaghi lab! nextPYP is a turn-key framework for single-particle cryo-ET that streamlines complex data analysis pipelines, from pre-processing of tilt series to high-resolution refinement, for efficient analysis of large datasets. nature.com/articles/s41592-0…
When you're at lunch and there's an atlas of the table on your leg. #SeeingCryoEMeverywhere 😅 #CryoEM
Jonathan Bouvette retweeted
happened IRL
Me: I’m a breastfeeding mom so carrying milk/ice packs frozen solid
Tsa: where is your baby
Me: not with me…which is why I have to pump
Tsa: well how we do know you’re really BF
Me: if the baby were w me I wouldn’t have to pump @momademia
Jonathan Bouvette retweeted
Great work by Anna-Sophia Krebs in collaboration with Juan Perilla and Alberto Bartesaghi's labs, demonstrating the molecular architecture and conservation of an immature human endogenous retrovirus
@OxfordStrubi @eBIC_Diamond
biorxiv.org/content/10.1101/…
Jonathan Bouvette retweeted
Our paper is out! Congratulations to @Cassandra_Hayne @TheHayneLab @RobinStanleyLab at @NIEHS ! I had fun image processing this project! nature.com/articles/s41594-0…
I was considering switching my SmartScope.org @djangoproject to a @JavaScript frontend until I discovered @htmx_org! I very much love to focus on writing python and have everything in one place.
Jonathan Bouvette retweeted
Great news our North Carolina Biotech Center Innovation Impact Grant (GII) was accepted. It's going to be amazing. @NCLifeSciences
med.unc.edu/biochem/news/cry…
SmartScope v0.9 collecting at a rate of 421 movies/h on R2/1, 7-shots per hole, very conservative 4.5um max beam-image shift.
Setup time after the scope was aligned: 20 min.
Fully automated from there.
😅Just need to improve the display of the targets at the medium mag now..
SmartScope v0.9 is out!
Highlights of the release are (details in thread):
- Multishot per hole
- Automatic focus position from hole lattice orientation and spacing.
- Redesigned docs website.
Join our Google group! (groups.google.com/g/smartsco…)
Simply provide a few information and SmartScope will suggest optimized multishot per hole pattern with some stats about total hole coverage and fraction of the images that is within the hole.
Smartscope Documentation now has its own repository github.com/NIEHS/SmartScope-…
Jonathan Bouvette retweeted
My in-person @ucdavis biochem class was "ZOOM-bombed" today by a structural biologist @GntlmnScientist from @UCBUSA. We made lemonade out of lemons. Students loved having you in class, David. (I stream via ZOOM to record & for those who have a hardship).
youtu.be/MG_TaVkt9ws
SmartScope now has a forum for discussion. Please join! groups.google.com/g/smartsco….
Oh ...and also multi-shot per hole with automated pattern is in beta.
Today I tried the Light ‘em up to set collection on a grid where i knew there were a lot of empty holes. It worked nicely! Look at all the crappy data I can skip! Thanks @WimJHH for the tool.
biorxiv.org/content/10.1101/…
Jonathan Bouvette retweeted
Almost 3 years later and 2.5 years with a child in daycare. Only took 1 mitochondria @GordonConf with no one masked (tested positive two days after returning).
@Momademia #WomenInSTEM #grc #mito #mitogrc
