
postdoctoral at @PD_lab_DDPSC studying 🌱cell-to-cell communication. NIH/NRSA fellow. Former NSF/GRFP & @UTKPEER Scholar #RITNTID alum #Science4ALL 🇳🇵🇸🇱🇺🇸
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ALT Results from the three methods of aniline blue staining for imaging clusters of plasmodesmata in Nicotiana benthamiana leaf epidermal cells with staining of plasmodesmal callose using 1% aniline blue solution. Representative images are shown for three different callose staining methods, both for untreated and salicylic acid (SA)-treated plants. A, Method 1 using fixed tissue, with white arrows indicating artifacts that are not within the cell wall. B, Method 1 in tissue treated with 0.05 mM SA for 12h. C, Method 2 using non-fixed tissue directly infiltrated with aniline blue. D, Method 2 in 0.05mM SA-treated tissues. E, Method 3 using non-fixed tissue stained with aniline blue for 30 to 60 min. F, Method 3 in 0.05mM SA-treated tissues.


























