@EltsovMikhail

DNA and chromatin in situ/ Cryo-ET @IGBMC

 Strasbourg, France
Joined October 2018
  • Tweets441
  • Following130
  • Followers342
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We are in EMBO J. See DNA and non-octameric nucleosome-like particles in situ link.springer.com/article/10…
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Mikhail Eltsov retweeted
Molecular Cell@MolecularCellSep 20
HP1α binding creates a local barrier against transcription activation and persists during chromatin decondensation dlvr.it/TVZ0MW
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Mikhail Eltsov retweeted
Kazuhiro Maeshima@kazu_maeshimaSep 15
🙏 Thanks to all contributors to our chromosome studies, especially those behind these works: 🧬cell.com/current-biology/ful… 🧬nature.com/articles/s41467-0… 🧬pnas.org/doi/abs/10.1073/pna… 🧬nature.com/articles/s41588-0…

A Transient Rise in Free Mg2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromos...

How the negatively charged long genomic DNA is organized into mitotic chromosome remains unclear. Using a newly developed Mg2+ indicator, Maeshima et al. demonstrate a transient rise in free Mg2+...

cell.com
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Mikhail Eltsov retweeted
Kazuhiro Maeshima@kazu_maeshimaSep 15
How are mitotic chromosomes built? 🏗️ Our Review @TrendsGenetics revisits the classical chromosome scaffold model and connects it to condensins, topoIIα, and physical forces. We propose interphase chromatin domains as “building blocks”🧩of chromosomes: authors.elsevier.com/a/1nmwr…
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Great work Kazu! So happy to see it published so high!
Kazuhiro Maeshima@kazu_maeshimaSep 8
Is euchromatin really “open”? 🧬 Using super-resolution imaging🔬 our new study @NatureGenet reveals: Euchromatin forms condensed domains in live cells. Cohesin constrains them and prevents domain mixing for proper transcriptional insulation🚧 🔗nature.com/articles/s41588-0… (1/2)
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Excellent work! Particularly intrigued by the observations on nucleolus-associated heterochromatin.
Kazuhiro Maeshima@kazu_maeshimaJul 7
Our new preprint @biorxivpreprint, led by @katsu_s_minami et al.! 🎉🧬🔬 biorxiv.org/content/10.64898… Machine learning-assisted Repli-Histo labeling and single-nucleosome imaging reveal distinct transcription-dependent chromatin constraints across euchromatin and heterochromatin.
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I am not afraid to say it: this is the most fundamental advance in our understanding of chromatin structure since the zig-zag fiber model.
Kazuhiro Maeshima@kazu_maeshimaApr 8
Replying to @kazu_maeshima
Using single-H1 imaging and computational modeling, we show that most H1 behaves like a liquid inside chromatin domains, rather than binding stably to nucleosomes as in the textbook model. Congrats, @MasaAShimazoe & all contributors! 🎉 2/2 🔗science.org/doi/10.1126/scia…
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Generate crowded synthetic tomographic data and train your model for particle picking in real data. Impressive work by Mohamad Harastani: nature.com/articles/s41467-0…
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Our new work @biorxivpreprint 🔗biorxiv.org/cgi/content/shor… BRD4-NUT forms liquid-like condensates that locally constrain nucleosomes via bromodomain-mediated crosslinking: physical control of #chromatin by #LLPS transcription condensates. @semeigazin @katsu_s_minami @MasaAShimazoe
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Mikhail Eltsov retweeted
Benoit Zuber@ben8080802 Jul 2025
A plain language overview of our recent publication link.growkudos.com/1e0cnguwx…

Near-atomic detail from large sample areas using cryo-EM of frozen biological sample slices

This study explores how cryo-electron microscopy (cryo-EM) can be used to image large areas of frozen biological samples with near-atomic resolution. The researchers used a method called CEMOVIS,...

growkudos.com
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Straightening FSC curves biorxiv.org/content/10.1101/… #biorxiv_biophys

Straightening FSC curves

The Fourier Shell Correlation (FSC) is traditionally calculated in resolution shells and plotted as a function of the inverse resolution. To ensure consistent statistics, these resolution shells...

biorxiv.org
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Mikhail Eltsov retweeted
Benoit Zuber@ben80808025 Jun 2025
Our latest paper is out. Getting high resolution reconstruction from vitreous cryo-sections.
IUCrJ@IUCrJ25 Jun 2025
Johannes Elferich et al.: In situ high-resolution cryo-EM reconstructions from CEMOVIS #ElectronTomography #IntegrativeStructuralBiology #Imaging @unibern... #IUCr journals.iucr.org/paper?S205…
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the first preprint of the lab! So excited! 😎#archaea #cryoEM #chromatin #Asgard
bioRxiv Biophysics@biorxiv_biophys26 May 2025
Cryo-EM reveals open and closed Asgard chromatin assemblies biorxiv.org/content/10.1101/… #biorxiv_biophys

Cryo-EM reveals open and closed Asgard chromatin assemblies

Asgards are the closest archaeal relatives of eukaryotes, representing an important step in chromatin evolution. However, their chromatin organization has remained enigmatic until now. In this study,...

biorxiv.org
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I am seeking an Assistant Professor in my lab. If you are interested, please contact me! 📩 ゲノムダイナミクス研究室で助教を公募中です。ご興味のある方はぜひご連絡ください！ RTお願いします。 🔄 日本語版👇👇👇
国立遺伝学研究所@NIG_idenken11 Mar 2025
【募集】助教 / ゲノムダイナミクス研究室 (PI: 前島一博) nig.ac.jp/nig/ja/2025/03/inf… #採用情報
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🧬My retrospective chromatin review is out@Proc Jpn Acad: doi.org/10.2183/pjab.101.020 Textbook models of 30-nm fibers are fading. A new paradigm: chromatin forms liquid-like domains—locally dynamic, yet globally stable at chromosome level (viscoelastic)—supporting genome function.✨
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🎥 Video: See chromatin in action — each dot is a single nucleosome, dynamically fluctuating in a living HeLa cell (TMR-labeled, 50 ms/frame). Original paper: science.org/doi/full/10.1126…

Single-nucleosome imaging reveals steady-state motion of interphase chromatin in living human cells

Local chromatin motion remains steady during interphase, allowing cells to conduct housekeeping tasks under similar environments.

science.org
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A question for the experts: Can you recognize nucleosomes and linker DNA in this data? Find an updated version of our manuscript in biorxiv.org/content/10.1101/…
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Spectacular work!
Kazuhiro Maeshima@kazu_maeshima21 Aug 2024
Excited to share our new paper @NatureComms. bit.ly/3WUkxbJ Single-nucleosome imaging reveals that condensins act as molecular crosslinkers, locally constraining nucleosomes to organize mitotic chromosomes. Nucleosome-nucleosome interactions constrain whole chromosomes.
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There is still time to apply for the cellular structural biology #postdoc position in my team @IGBMC. This is your opportunity to acquire/further hone your cryo-ET skills ❄️
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Mikhail Eltsov retweeted
Valerie Lamour@valeroche14 Apr 2024
DNA gyrase captures a positive crossover prior to taking action doi.org/10.1126/science.adl5… @Naveen_Kumar__M @marechal_nils @LynnZechiedrich @instructhub @BioS_ME_Alsace @IGBMC @IBENS_ENS @Inserm @BCMFromtheLabs @CNRSbiologie @unistra @univ_paris_cite
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