@Jake_T_Bush

Chemical biologist at GSK and the Francis Crick Institute.

 London, England
Joined July 2012
  • Tweets78
  • Following202
  • Followers254
7Media
Synthesis without purification still yields clear fragment hits.  'Direct-to-Biology' screens crude reaction mixtures & yields covalent and noncovalent inhibitors of SARS-CoV-2 main protease practicalfragments.blogspot.… @angew_chem @Jake_T_Bush @GSK
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Excited to share a key part of my postdoctoral work from @TheCrick and @GSK , published in @NatureComms . A collaborative project to develop a high-throughput chemoproteomics platform for profiling cysteine-reactive fragments in native biological systems. nature.com/articles/s41467-0…

Robust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics

Nature Communications - This paper presents a label-free chemoproteomics platform using data-independent acquisition to profile covalent fragment binding across the human proteome. The platform...

nature.com
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Rapid optimisation of covalent and non-covalent SARS MPro inhibitors by ‘direct-to-biology’ reactive fragment screening. Great work by Harry Wilders on @ChemRxiv from @GSK, @UniStrathclyde and @TheCrick. #chembio chemrxiv.org/engage/chemrxiv…

Expedited SARS-CoV-2 main protease inhibitor discovery through modular ‘direct-to-biology’ screen...

Reactive fragment (RF) screening has emerged as an efficient method for ligand discovery across the proteome, irrespective of a target’s perceived tractability. To date, however, the efficiency of...

chemrxiv.org
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I'm delighted to share our recent article developing an approach to identify cyclic peptide binders of challenging protein targets directly in cell lysates - led by @catherine_hurd with GSK's @Jake_T_Bush and Andy Powell @TheCrick @impch
Angewandte Chemie@angew_chem15 Aug 2024
mRNA Display in Cell Lysates Enables Identification of Cyclic Peptides Targeting the BRD3 Extraterminal Domain (Louise J. Walport and co-workers) @lwalport #openaccess onlinelibrary.wiley.com/doi/…

mRNA Display in Cell Lysates Enables Identification of Cyclic Peptides Targeting the BRD3 Extrate...

A method to perform mRNA display selections against protein targets expressed in mammalian cell lysates has been developed. This approach circumvents the need for purified proteins to be produced,...

onlinelibrary.wiley.com
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Fantastic work by George, Emma and the team in the @GSK and @TheCrick collaboration: a robust chemoproteomics platform to screen covalent compounds against the proteome. Exciting work on-going to deploy at scale with AI/ML to map the druggable proteome. biorxiv.org/content/10.1101/…
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Hot off the press! Check out our ‘mutate and conjugate’ paper in @ACSBioMed 🔥 Here we demonstrate that combining site-directed mutagenesis and reactive fragments can be used to rapidly identify selective covalent probes for target validation studies.
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Our 'Mutate and Conjugate' paper is out now in ACS Chem Biol (@ACSBioMed)! We combine site-directed mutagenesis and electrophilic fragments to identify inhibitors the mutant protein with a view to helping target validation. pubs.acs.org/doi/10.1021/acs… #chembiol #medchem
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Happy to share our latest preprint. Catherine used #biochemical & #structural methods to study the #epigenetic reader protein BDF5 as a potential drug target in #Leishmania. Partnership between @Mottram_group , Wilkinson lab @YSBL_York & @conway_group. biorxiv.org/cgi/content/shor…
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Check out recent work by @Jake_T_Bush, Nicholas Tomkinson & co: 'Reactive fragments targeting carboxylate residues employing direct to biology, high-throughput chemistry' The #OpenAccess article is online now🔓 pubs.rsc.org/en/content/arti… 📍 @GSK and @UniStrathclyde

Reactive fragments targeting carboxylate residues employing direct to biology, high-throughput...

The screening of covalent or ‘reactive’ fragment libraries against proteins is becoming an integral approach in hit identification, enabling the developmen

pubs.rsc.org
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Excited to share the latest publication from our collaboration between @GSK and @TheCrick. Expanding the liganded DUBome to support target validation in biomedical research. Great work from Rosa Cookson, Aini Vuorinen and co-authors. #chembio @target2035 biorxiv.org/content/10.1101/…

A chemoproteomic platform for reactive fragment profiling against the deubiquitinases

Chemoproteomics is a powerful method capable of detecting interactions between small molecules and the proteome, however its use as a high-throughput screening method for chemical libraries has so...

biorxiv.org
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Our latest paper in collaboration with @GSK describes how the reactivity of a series of SuFEx reagents influences covalent modificaiton of the proteome. Congratulations to @KateGilbert_ , @Jake_T_Bush and @arronaatkar . pubs.acs.org/doi/10.1021/acs…

Profiling Sulfur(VI) Fluorides as Reactive Functionalities for Chemical Biology Tools and Expansion...

Abstract. Here, we report a comprehensive profiling of sulfur­(VI) fluorides (SVI-Fs) as reactive groups for chemical biology applications. SVI-Fs are reac

pubs.acs.org
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Efficient Ligand Discovery Using Sulfur(VI) Fluoride Reactive Fragments [@arronaatkar] dlvr.it/SfJZpN
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Check out @arronaatkar’s work taking reactive fragments into new landscapes: beyond-cysteine covalent ligand discovery with sulfonyl fluorides @ChemRxiv. Great collab @GSK, @StrathChem, @TheCrick. #chemicalbiology #covalentmodifiers (Artwork @emma_k_grant) lnkd.in/eec64JRZ
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Excited to share this opportunity for a bioinformatics post doc in the Crick-GSK collaboration linkedin.com/posts/activity-…
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Great opportunity to join the @TheCrick / @GSK partnership to build systems chemical biology approaches to therapeutic discovery. #chemicalbiology #chembio #covalentmodifiers
J. Mark Skehel@SkehelJM11 Aug 2022
We're recruiting for the Crick/GSK EPSRC prosperity partnership! If you're thinking about a postdoctoral position have a look at the link below! @TheCrick crick.ac.uk/careers-study/va…
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We're recruiting for the Crick/GSK EPSRC prosperity partnership! If you're thinking about a postdoctoral position have a look at the link below! @TheCrick crick.ac.uk/careers-study/va…
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Register now for our free #RSCDesktopSeminar Lectureship webinar, featuring Lectureship winners @Jake_T_Bush & @london_lab! 📅 4 Aug 2022 at 1 PM BST Even if you can't attend on the day, be sure to register to receive a recording of the webinar later! rsc.zoom.us/webinar/register…
Graphic showing details of the webinar and photos of speakers Dr Jacob Bush and Dr Nir London

ALT Graphic showing details of the webinar and photos of speakers Dr Jacob Bush and Dr Nir London

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Our #RSCDesktopSeminar Lectureship is TODAY! 🎉 Register now to see talks by our Lectureship winners, Dr Jacob Bush (@Jake_T_Bush) and Dr Nir London (@london_lab) at 1 PM BST ➡ rsc.zoom.us/webinar/register…
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One week until our free #RSCDesktopSeminar Lectureship webinar, featuring our 2021 & 2022 winners, Dr Jacob Bush (@Jake_T_Bush) and Dr Nir London (@london_lab)! ✨ 📅 4 August 2022 at 1 PM BST 🔗 bit.ly/3NZlt8j
Graphic showing information about webinar

ALT Graphic showing information about webinar

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Interesting paper by the groups of @Yang_J_lab and Ping Xu in @JProteomeRes. They directly compared five different cleavable biotin tags and 3 different workflows to characterize their performance in residue-specific chemoproteomics. doi.org/10.1021/acs.jproteom…

Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics

Abstract. Click chemistryspecifically the copper-catalyzed azide-alkyne cycloadditionhas enabled the development of a wide range of

pubs.acs.org
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