@LabNikel

Building synthetic metabolisms in bacteria for a sustainable future @DTUBiosustain · Views are our own + #Pseudomonas’ 🧬 ·

 Copenhagen, Denmark
sem-cfb.com
Joined August 2018
  • Tweets793
  • Following348
  • Followers1.9K
225Media
Seven Critical Challenges in Synthetic One-Carbon Assimilation and Their Potential Solutions—led by @FavoinoGiusi & @OscarPuiggene @LabNikel @FEMSmicro #Microbiology #Reviews #SynBio #Metabolic #Engineering academic.oup.com/femsre/adva…
11
1
32
2,525
Ins and outs for designing bacterial metabolisms for C1-assimilation, from a multilevel perspective, now pieced together in this review from our @LabNikel! Check it out at academic.oup.com/femsre/adva…

Seven critical challenges in synthetic one-carbon assimilation and their potential solutions

In this review, we summarize the challenges in establishing synthetic pathways for assimilation of C1 feedstocks in microbes and how to solve these issues

academic.oup.com
Pablo Iván Nikel @pabnik.bsky.social@PabloINik3 Apr 2025
Seven Critical Challenges in Synthetic One-Carbon Assimilation and Their Potential Solutions—led by @FavoinoGiusi & @OscarPuiggene @LabNikel @FEMSmicro #Microbiology #Reviews #SynBio #Metabolic #Engineering academic.oup.com/femsre/adva…
3
9
692
Really happy to share that our work on constructing six glyoxylate / glycolate sensors covering a three order of magnitude concentration range is out now in @NatureComms ! 🔥 Read below to see how we developed the sensors and what they are good for 🥳. nature.com/articles/s41467-0…

Computation-aided designs enable developing auxotrophic metabolic sensors for wide-range glyoxylate...

Nature Communications - Auxotrophic metabolic sensors (AMS) are vital for bioengineering but are often time-consuming to develop. Here, the authors present a workflow for designing versatile AMS,...

nature.com
9
8
3
29
1,959
Preprint alert🚨Systematic #engineering of synthetic serine cycles in #Pseudomonas putida uncovers emergent topologies for #methanol assimilation · @biorxivpreprint led by @OscarPuiggene #SynBio #Methylotrophy #CircularEconomy @LabNikel biorxiv.org/content/10.1101/…

Systematic engineering of synthetic serine cycles in Pseudomonas putida uncovers emergent topolog...

The urgent need for a circular carbon economy has driven research into sustainable substrates, including one-carbon (C1) compounds. The non-pathogenic soil bacterium Pseudomonas putida is a promising...

biorxiv.org
8
1
27
1,785
Super exciting work by @OscarPuiggene from @LabNikel on advancing #methanol use by Pseudomonas Putida by engineering #serinecycle variants! Many congratulations, excited to be a part of this !
Pablo Iván Nikel @pabnik.bsky.social@PabloINik21 Feb 2025
Preprint alert🚨Systematic #engineering of synthetic serine cycles in #Pseudomonas putida uncovers emergent topologies for #methanol assimilation · @biorxivpreprint led by @OscarPuiggene #SynBio #Methylotrophy #CircularEconomy @LabNikel biorxiv.org/content/10.1101/…

Systematic engineering of synthetic serine cycles in Pseudomonas putida uncovers emergent topolog...

The urgent need for a circular carbon economy has driven research into sustainable substrates, including one-carbon (C1) compounds. The non-pathogenic soil bacterium Pseudomonas putida is a promising...

biorxiv.org
2
13
491
Engineering a phi15-based #expression system for stringent #gene expression in #Pseudomonas putida—led by @EvelineLammens, @Daniel__Volke in a nice collab between @LabNikel & @RobLavigne1's team nature.com/articles/s42003-0…

Engineering a phi15-based expression system for stringent gene expression in Pseudomonas putida

Communications Biology - The development of a modular, phage-based expression system optimized for Pseudomonas putida enables stringent, tunable and high yield expressions with minimal cytotoxicity.

nature.com
8
32
1,599
CIFR (Clone–Integrate–Flip-out–Repeat): A toolset for iterative #genome and pathway #engineering of Gram-negative #bacteria—our latest contribution led by @FilFederici & Francesco Luppino @LabNikel @DTUBiosustain #OpenAccess #SynBio sciencedirect.com/science/ar…
10
1
61
2,775
Amazing tool for multiple cycles of genetic engineering of Gram negative bacteria developed by @FilFederici and Francesco, now fully available! @LabNikel
Pablo Iván Nikel @pabnik.bsky.social@PabloINik17 Jan 2025
CIFR (Clone–Integrate–Flip-out–Repeat): A toolset for iterative #genome and pathway #engineering of Gram-negative #bacteria—our latest contribution led by @FilFederici & Francesco Luppino @LabNikel @DTUBiosustain #OpenAccess #SynBio sciencedirect.com/science/ar…
1
3
247
Leveraging engineered #Pseudomonas putida minicells for bioconversion of organic acids into short-chain methyl ketones by @KaterinaKozaeva, @ManuelNietoDom1, @maxi_prep et al. @ACSSynBio @LabNikel @DTUBiosustain #OpenAccess pubs.acs.org/doi/10.1021/acs…

Leveraging Engineered Pseudomonas putida Minicells for Bioconversion of Organic Acids into Short-...

Abstract. Methyl ketones, key building blocks widely used in diverse industrial applications, largely depend on oil-derived chemical methods for their prod

pubs.acs.org
1
10
53
2,085
A fine example of employing rationally - and evolved - engineered bacteria to sense diverse compounds from our @LabNikel, great work @Javi_HernanSan and @Daniel__Volke!
Pablo Iván Nikel @pabnik.bsky.social@PabloINik27 Sep 2024
A versatile #microbial platform as a tunable whole-cell #chemical #sensor | #Pseudomonas putida engineered to detect a variety of molecules (including #sensing #plastic #degradation) by @Javi_HernanSan @Daniel__Volke et al. @LabNikel @DTUBiosustain nature.com/articles/s41467-0…

A versatile microbial platform as a tunable whole-cell chemical sensor

Nature Communications - Biosensors are powerful tools for quantification of a wide range of molecules but require extensive engineering for each analyte. Here, the authors engineered a robust...

nature.com
1
4
338
#GCSB2024 #Regensburg was a fantastic conference! Thanks @gasb_synbio & @DECHEMA for the invitation & efficient organization👏🏼👏🏼It was great to catch up w/the German #SynBio community & present our latest results @LabNikel @DTUBiosustain #Pseudomonas #microbial #engineering
GASB SynBio@gasb_synbio20 Sep 2024
And with that, its a wrap🎉🎉🎉! #GCSB2024 is officially over and we had an absolute blast during this conference! We whole-heartedly thank everyone involved, especially our colleagues at @DECHEMA, GFGSB and the location @MarinaForum🫶!
1
18
1,221
Another talk from the @LabNikel on #Pseudomonas2024. @FilFederici presents a novel toolset he developed for iterative genome engineering of Pseudomonas, wonderful job! (stay tuned for the published work) @PseudomonasC @MicrobioSoc
3
14
1,106
Amazing talk by our @OscarPuiggene from @LabNikel on exploiting and engineering C1-assimilation cycles to achieve synthetic methylotrophy in Pseudomonas! @PseudomonasC @MicrobioSoc #Pseudomonas2024
4
1
10
3,167
And... We are off to a great start of #Pseudomonas2024! Meet @mgracialvan, @Javi_HernanSan & @FavoinoGiusi @LabNikel at the front desk. Registration is now open! @MicrobioSoc @PseudomonasC 🦠🧫
1
6
84
4,608
Part of the @LabNikel gang at the #Pseudomonas2024 opening! Check the work (posters & talks) by @OscarPuiggene, @mgracialvan, @FavoinoGiusi, Emil C. Ørsted, @FilFederici & @MichelePartipi3! #ProudPI
7
18
1,846
Thanks for the invite @Gonnyita @rafgir63 @jorgebarriuso German Rivas et al. @UIMP @CSIC, it's been a blast to interact w/students & colleagues while discussing our work at @LabNikel @DTUBiosustain #SynBio #Metabolic #Engineering
Gonzalo Durante@Gonnyita26 Aug 2024
Comienzan las sesiones científicas de la X Escuela de Biología Sintética Integrativa y abre nuestro querido @PabloINik poniendo de relieve la problemática del calentamiento global y algunas soluciones propuestas desde la Biotecnología @UIMP @CSIC
4
17
1,172
When a member leaves the @LabNikel SEM team, they receive a personalized gift to remember their time with us at @DTUBiosustain. Johannes Büchele, as passionate about #fluorine #biochemistry as he is about #bouldering, proudly uses his SEM-customized bouldering brush in Austria!🏔️
1
31
2,038
Fluorine biocatalysis expanded to produce fluorinated D-amino acids and trifluorinated variants thereof, reported by @PabloINik, @ManuelNietoDom1, @LabNikel, @NovoNordiskFond @DTUBiosustain nature.com/articles/s42004-0…

Enzymatic synthesis of mono- and trifluorinated alanine enantiomers expands the scope of fluorine...

Communications Chemistry - Establishing biotechnological alternatives to chemical syntheses requires the rational design of biosynthetic pathways and degradation routes either as enzymatic cascades...

nature.com
2
3
892
#Enzymatic #synthesis of mono- and trifluorinated alanine enantiomers expands the scope of #fluorine #biocatalysis ― led by @ManuelNietoDom1 w/Abou @LabNikel & colleagues @DTUKemi @CHGotfredsen #OpenAccess @CommsChem nature.com/articles/s42004-0…
1
9
48
3,254
"Advanced #SynBio for Creating Sustainable Cell Factories", led by @Daniel__Volke & @damgaard_jensen, runs @DTUBiosustain on June 6th-26th · Several hosts (yes, including #Pseudomonas!), in silico-driven design & hands-on experiments included! Apply here: kurser.dtu.dk/course/29902
10
1
24
3,551
Load more