@Lew_Lab

Playing with #singlemolecules, bending light, and algorithms to see really small things. Led by Matthew Lew, Associate Professor @WashUESE @WashUengineers

 St Louis, MO
lewlab.wustl.edu
Joined October 2015
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  • Following152
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It's great to see Alex's undergraduate work on optimal excitation polarization modulation now out in #BOE. He will be joining us as an #ImagingScience PhD student in the New Year! doi.org/10.1364/BOE.607950
WashU McKelvey Engineering@WashUengineersSep 14
Replying to @WashUESE
@WashUESE alum Alex Nie with Matthew Lew found that polarized light patterns can improve the precision of microscopes used to measure the orientation of single molecules. engineering.washu.edu/news/2…

How light can reveal the hidden orientation of a single molecule

engineering.washu.edu
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We made the cover! Check out Weiyan's work exploring the "Fundamental Limits in Measuring the Anisotropic #RotationalDiffusion of #SingleMolecules" in @JPhysChem doi.org/10.1021/acs.jpca.4c0… @WashUESE @WashUengineers
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Brian's study on using the #binding #orientations of Nile blue to decipher the architectures of growing, decaying, and remodeling #amyloid #fibrils is now online in @NanoLetters ! #singlemolecule @WashUESE @WashUengineers doi.org/10.1021/acs.nanolett…
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Happy to see @Tingting_Wu_'s review chapter on 6D dipole-spread function engineering now online doi.org/10.1007/978-3-031-39… @WashUengineers @WashUESE A big thanks to @JinyangLiang for putting such a great volume together! doi.org/10.1007/978-3-031-39…
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Our work has made the inside cover! Check out "#singlemolecule #electrochemical imaging resolves the midpoint potentials of individual fluorophores..." in the latest issue of @ChemicalScience! doi.org/10.1039/d3sc05293a @WashUengineers @WashUESE
Showcasing research from Professor Matthew D. Lew's laboratory, McKelvey School of Engineering, Washington University in St. Louis, MO, USA. Single-molecule electrochemical imaging resolves the midpoint potentials of individual fluorophores on nanoporous antimony-doped tin oxide. See Jin Lu and Matthew D. Lew, Chem. Sci. 2024, 15, 2037.

ALT Showcasing research from Professor Matthew D. Lew's laboratory, McKelvey School of Engineering, Washington University in St. Louis, MO, USA. Single-molecule electrochemical imaging resolves the midpoint potentials of individual fluorophores on nanoporous antimony-doped tin oxide. See Jin Lu and Matthew D. Lew, Chem. Sci. 2024, 15, 2037.

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Congratulations to Prof. Jin Lu, now at the National Center for Nanoscience, whose work on single-molecule electrochemical imaging is now online in @ChemicalScience! #MyFirstChemSci doi.org/10.1039/D3SC05293A
Single-molecule electrochemical imaging resolves spatially varying midpoint potentials on a transparent electrode

ALT Single-molecule electrochemical imaging resolves spatially varying midpoint potentials on a transparent electrode

Nanoscale Electrochemistry and Imaging Papers@NanoECimaging29 Dec 2023
Single-Molecule Electrochemical Imaging Resolves the Midpoint Potentials of Individual Fluorophores on Nanoporous Antimony-Doped Tin Oxide pubs.rsc.org/en/Content/Arti…

Single-molecule electrochemical imaging resolves the midpoint potentials of individual fluorophores...

We report reversible switching of oxazine, cyanine, and rhodamine dyes by a nanoporous antimony-doped tin oxide electrode that enables single-molecule (SM)

pubs.rsc.org
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Join us for @Tingting_Wu_'s PhD defense, "Information-Rich #6D #SingleMolecule Imaging Using Optimization," tomorrow at 1 PM CST in Rodin Auditorium! happenings.wustl.edu/event/e… @WashUESE @wuimgsci_stuco @wustlspectra @WashUengineers
Information-Rich 6D Single-Molecule Imaging Using Optimization Tingting Wu Program in Imaging Science Laboratory of Dr. Matthew D. Lew Department of Electrical & Systems Engineering Monday, November 20, 2023 Green Hall, Rodin Auditorium 1:00 p.m. (CT)

ALT Information-Rich 6D Single-Molecule Imaging Using Optimization Tingting Wu Program in Imaging Science Laboratory of Dr. Matthew D. Lew Department of Electrical & Systems Engineering Monday, November 20, 2023 Green Hall, Rodin Auditorium 1:00 p.m. (CT)

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#bps2023 Come see @Tingting_Wu_ tell a beautiful story on “Mapping Inhomogeneous Network Structures within Biomolecular Condensates using Fluorogenic Probes,” tomorrow at 9:15 AM in Room 6C, a collaboration with @PappulabWashU
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We'll also be presenting “A Multi-View Reflector Microscope Visualizes Six-Dimensional Single-Molecule Translational And Rotational Dynamics With Isotropic Nanoscale Resolution!” Poster 1351-Pos / B480 doi.org/10.1016/j.bpj.2022.1…
Lipid-coated silica spheres as visualized by 6D images of Nile red

ALT Lipid-coated silica spheres as visualized by 6D images of Nile red

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#bps2023 Come hear Brian talk about “Single-Molecule Orientation-Localization Microscopy Resolves How Amyloidophilic Dye Orientations are Correlated with Amyloid Fiber Growth and Disruption,” Poster 1339-Pos / B468 tomorrow at 1:45 PM! doi.org/10.1016/j.bpj.2022.1…
Amyloid-beta fiber remodeling, observed by standard localization microscopy and single-molecule orientation-localization microscopy

ALT Amyloid-beta fiber remodeling, observed by standard localization microscopy and single-molecule orientation-localization microscopy

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#bps2023 Come visit Weiyan tomorrow as she presents “#SingleMolecule Orientation-Localization Microscopy Resolves the Nanoscale Organization of Self-Assembled Peptides,” Poster 627-Pos / B448 at 1:45 PM in Exhibit Hall BC doi.org/10.1016/j.bpj.2022.1…
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Happy to share new collaborative work on #singlemolecule imaging of biomolecular condensates. They're less uniform than they seem! With @PappulabWashU @Tingting_Wu_ @MattKingLab @MinaFaragSci @WashUengineers
Epifluorescence imaging shows that fluorogenic probes sense chemical environments inside biomolecular condensates from different perspectives. As fluorogenic probes randomly translate through solution, they encounter various chemical environments formed by the low-complexity domain of the hnRNPA1 protein in the dilute phase or in the condensate. In response, they either remain dark or become fluorescence emitters.

ALT Epifluorescence imaging shows that fluorogenic probes sense chemical environments inside biomolecular condensates from different perspectives. As fluorogenic probes randomly translate through solution, they encounter various chemical environments formed by the low-complexity domain of the hnRNPA1 protein in the dilute phase or in the condensate. In response, they either remain dark or become fluorescence emitters.

bioRxiv Biophysics@biorxiv_biophys28 Jan 2023
Single fluorogen imaging reveals spatial inhomogeneities within biomolecular condensates biorxiv.org/cgi/content/shor… #biorxiv_biophys
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We were absolutely inspired by your annular mirror work from all those years ago @HohlbeinLab ! Emission dipole physics is cool!
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Excited to share that our 6D #singlemolecule #superresolution microscope, which we uses a multi-view reflector architecture, is now online in @NaturePhotonics ! Congratulations @oumeng_zhang ! doi.org/10.1038/s41566-022-0…
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Partnering with @wustlspectra to demo imaging and optics at @STLScienceCtr #SciFest
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And we're off, Welch Conference "#singlemolecules and sculpted light"
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