Pinned Tweet
Pinning this to help convert between multiple alternative numbering systems in use for influenza H5N1 hemagluttinin.
Replying to @jbloom_lab
In addition, we have made a page to help convert between the many confusing numbering schemes used to name HA mutations: dms-vep.org/Flu_H5_American-…
(Throughout we use H3 numbering, but other studies use other schemes)
Very grateful to @sergeilkp ⭐
Sure loads of influenza experts are all over both
SHARED
PB2 Q13R
PB1 K388R
PA I129T
M2 G58D & S64F
and
later-DERIVED
PB2 I399T
PB1 N642T
NS1 T60A (rev)
Mutations in the Utah mink?
@E_A_Karlsson
@HNimanFC
@SolidEvidence
@RajlabN
Replying to @Nucleocapsoid @SolidEvidence
The 10 Utah mink genomes (26G11429, nested inside a May/June 2026 ID/UT cattle sub-branch) form a three-step internal ladder on the tree:
The basal mink branch (shared by all 10 mink tips, and absent in the sister ID/UT cattle tips) carries PB2 Q13R, PB1 K388R, and PA I129T, along with M2 G58D and S64F.
Two basal mink tips (-004 and -008, which has private PB1 S384L) branch off first, while the other 8 mink tips share an internal branch with PB2 I399T and the NS1 T60A reversion. A nested branch of 5 tips then adds PB1 N642T (plus singleton PA I465T and PB2 A661T). That branching order is consistent with PB2 I399T, NS1 T60A, and PB1 N642T arising during the mink outbreak after the initial introduction.
On the Oct 2026 Washington human sequence (EPI_ISL_205571770): per the GISAID release note, it clusters with this same 2026 ID/UT B3.13 branch and carries the shared NA substitution. It is missing from the Nextstrain cattle tree because CDC deposited this taxpayer-funded US sequence in GISAID (a non-US access-restricted database) rather than NCBI GenBank, so open pipelines built on GenBank/SRA cannot include it yet.
Euan Arnott retweeted
This is exactly the scenario that @DrFiliatrault, @TRyanGregory and I warned about in a Scientific American article last summer:
scientificamerican.com/artic…
We weren’t the only ones. This is such a predictable disaster unfolding in slow motion.
There is something really concerning (and disappointing) going on in the US right now.
A dangerous and preventable situation.
Let me explain.
nextstrain.org/avian-flu/h5n…
1/
Euan Arnott retweeted
I spent some time tonight looking at the Nextstrain H5N1 dairy cattle tree that @SolidEvidence shared. What jumps out at me is not an uncontained national wave, but a dramatic evolutionary bottleneck.
Every major 2024 and 2025 lineage went extinct. The massive California wave vanished last fall. So did the Colorado and Midwest lineages, along with the original Texas and Michigan clades. Every single 2026 sequence on that tree traces back to one late-2025 ancestor in Idaho.
Bulk milk testing worked. Mandatory bulk-tank screening and farm quarantines in Colorado and California, followed by the USDA National Milk Testing Strategy, cleared the virus from most dairy states. That state-by-state clearance is why the January 2026 USDA guidance added an exemption for certified unaffected states. Rather than circulating across the country, roughly 96% of the 2026 sequences come from two neighboring states, Idaho and Utah, plus one small secondary jump to Texas.
Why does the 2026 cluster sit on such a long branch? Sampling cadence explains a lot of the visual gap. Sequencing in Idaho went quiet for five months in late 2025 and then ramped back up this spring with much faster GenBank turnaround. Even with uneven sampling, the fact that every 2026 sequence across cattle, birds, and mink coalesces to a single late-2025 Idaho ancestor points to a real contraction.
We ran our selection, clock, and reassortment tools across all eight segments to see what carried that lone Idaho lineage through the bottleneck. Reassortment played no role. All eight segments tracked together clonally, and PB2 did not pick up a single nucleotide change along the entire bottleneck stem. The other seven segments went through a sharp clock acceleration and fixed a tight cluster of co-evolving changes in NS1, HA, and NA. When that lineage spilled into a Utah mink farm, it picked up a set of polymerase changes and immediately reverted an NS1 mutation (A60T) that the virus had acquired in Idaho cattle back in 2024.
Inside the surviving Idaho and Utah pocket, peridomestic birds and small mammals sit interleaved with dairy cattle on the tree. Curious what @SolidEvidence and others think: is this last lineage holding on in the Intermountain basin through local farm-to-wildlife cycling, or did those NS1 and surface changes alter within-herd persistence?
xitter.cf/SolidEvidence/status/2…
There is something really concerning (and disappointing) going on in the US right now.
A dangerous and preventable situation.
Let me explain.
nextstrain.org/avian-flu/h5n…
1/
Euan Arnott retweeted
nice article from Propublica describing an influenza H5N1 outbreak going on at a Utah mink farm. Situations like these pose more of a threat for development of better mammalian transmission yet government officials seem unable or unwilling to end it. propublica.org/article/bird-…
Please read this
I also suggest re-sharing (the original, not this Post of mine)
Too few people know about this in enough detail to be as worried as I am
There is something really concerning (and disappointing) going on in the US right now.
A dangerous and preventable situation.
Let me explain.
nextstrain.org/avian-flu/h5n…
1/
Euan Arnott retweeted
There is something really concerning (and disappointing) going on in the US right now.
A dangerous and preventable situation.
Let me explain.
nextstrain.org/avian-flu/h5n…
1/
Euan Arnott retweeted
Ebola update:
- Cases: 9,907 (+173)
- Recovered: 2,665 (+51)
- Deaths: 4,807 (+117)
Euan Arnott retweeted
This is basically a classical dangerous Gain of Function (dGoF) experiment. I wish the US government would shut it down.
A while ago, Stacey Schultz-Cherry and I wrote a commentary (pmc.ncbi.nlm.nih.gov/article…), that ends with this sentence:
US: Bird flu has been spreading for around two months on a Utah mink farm holding thousands of animals. None have been culled, workers aren't being monitored, and the USDA says it doesn't regulate mink. So thousands of animals are exposed, workers could be at risk, and nobody seems to be taking responsibility.
theguardian.com/us-news/2026…
Euan Arnott retweeted
What a beautiful video.
A mother and calf fell into a water tank. Both were rescued but during rescue the calf got separated from mother. Kurseong division staff brilliantly tracked the mother. It took 7 hours but they reunited them.
Reaction of mother in last !! Gods work.
I give up...
@KinCONN
Replying to @propublica
7/ Price also told us she has never set foot on the property. "They're a very private bunch," she said.
So last Saturday, we tracked down two of the farm’s workers ourselves.
One had a visibly red eye — a symptom present in over 90% of bird flu cases in humans.
Euan Arnott retweeted
Replying to @ZdenekVrozina
Yes, this is a great study. Complements this other 2026 study on ORF3a and cholesterol that in some ways might appear contradictory to the other, but which really just looks at a different aspect of how ORF3a affects cholesterol.
cell.com/cell-reports/fullte…
This would be my thinking too
Replying to @sergeilkp @nizzaneela
Good to know. Implausible to me that muts unique in sarbecos & known to be meaningful, like PLpro substrate-altering ORF1a:1795K, glycan-removing/conformation-changing S:372A, s2m-damaging 27958T—which all revert in Cryptic WW variants—weren’t pre-adaptations in intermediate host
Did I miss the whole Idaho emu farm H5N1 story?!
Ostriches I knew about, but have no recollection of emus in Summer 2026?
@MegCattell
@JusDayDa
@KinCONN
@RajlabN
@SolidEvidence
@ryan
Euan Arnott retweeted
Here’s something you should know about minks:
They have lungs and airways remarkably similar on a cellular level to those of people.
They’ve spread flus and infected humans before with COVID-19.
Right now, bird flu is spreading on a Utah farm filled with thousands of them… 🧵
US airport sampling (CDC):
Initial "KP.3" labelling of PJ.2.1 in airline arrival swabs is of course understandable given that PJ.2.1 is an off-shoot of KP.3.1.1 via evolution for a year or so in a chronic (likely human) host
Kanto glows....
but ONLY when you turn up the *brightness*
Tail of post-vaccation BA.3.2 "return wave" in Japan (and start of PQ.16.1.1, etc. wavelet) has been a subdued affair so far
Population immunity is holding up quite well in Japan, and increasing inter-infection gaps
Washington State human case of H5N1 linked to their 9-herd dairy outbreak is indeed B3.13
("standard" dairy strain of High Path avian influenza which emerged in Texas in Spring 2024)
Ironically TX is the other *current* H5N1 dairy hotspot, with Kansas also involved
@hnim
AFluDiary Blog `Texas & Washington Report More #H5N1 in Dairy Cows & #FluView Wk 41 Update' afludiary.blogspot.com/2026/…





























