Dr. Rhys Livingstone (@RhysLivingstone) header
Dr. Rhys Livingstone (@RhysLivingstone) profile picture

Dr. Rhys Livingstone

@RhysLivingstone

Postdoc at the University of Edinburgh researching protein synthesis and SynGAP1 using super-resolution and live-cell microscopy

 Edinburgh, Scotland
loop.frontiersin.org/people/771077/overview
Joined May 2019
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Photo by Dr. Rhys Livingstone (@RhysLivingstone)Photo by Dr. Rhys Livingstone (@RhysLivingstone)Photo by Dr. Rhys Livingstone (@RhysLivingstone)Photo by Dr. Rhys Livingstone (@RhysLivingstone)Photo by Dr. Rhys Livingstone (@RhysLivingstone)Photo by Dr. Rhys Livingstone (@RhysLivingstone)Photo by Dr. Rhys Livingstone (@RhysLivingstone)Photo by Dr. Rhys Livingstone (@RhysLivingstone)Photo by Dr. Rhys Livingstone (@RhysLivingstone)Photo by Dr. Rhys Livingstone (@RhysLivingstone)
Back from the easter break and happy to say my second first-author publication has been published in @FrontNeurosci, following on from past research this work examined #sAPPa-mediated calcium-permeable #AMPAR trafficking and #LTP frontiersin.org/articles/10.…
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To the day, two years later, we have published our preprint describing what we found Contrary to the current model, SynGAP1 dispersion appears to be largely a GFP-tagging artefact. Instead, SynGAP1 may gate plasticity via translational repression. biorxiv.org/content/10.64898…
Local translational repression and retention of SynGAP1 during synaptic plasticity

Local translational repression and retention of SynGAP1 during synaptic plasticity

Synaptic plasticity depends on tightly controlled protein production, yet most locally synthesised synaptic proteins studied to date promote strengthening. Here, we investigate whether SynGAP1—a key...

biorxiv.org
Dr. Rhys Livingstone (@RhysLivingstone) profile pictureDr. Rhys Livingstone@RhysLivingstone3 May 2024
This #FluorescenceFriday is our first attempt at imaging SynGAP in live hippocampal neurons during a cLTP protocol Thanks to @rhuganir and @yoichi_araki for their beautifully expressing (and dispersing!) SynGAP construct!
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Dr. Rhys Livingstone retweeted
Paul Donlin-Asp 🏳️‍🌈 (@PDA_PhD) profile picture
Paul Donlin-Asp 🏳️‍🌈@PDA_PhDMay 1
Extremely excited to share the first preprint from the lab, led by postdoc @RhysLivingstone biorxiv.org/content/10.64898… What did he find? 1/7
Local translational repression and retention of SynGAP1 during synaptic plasticity

Local translational repression and retention of SynGAP1 during synaptic plasticity

Synaptic plasticity depends on tightly controlled protein production, yet most locally synthesised synaptic proteins studied to date promote strengthening. Here, we investigate whether SynGAP1—a key...

biorxiv.org
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I couldn’t be more proud. If anyone is looking for a very talented scientist to brighten up their lab, get in touch with Court 🎉
Courteney Westlake (@CourteneyMW) profile pictureCourteney Westlake@CourteneyMW18 Dec 2024
🍾Beyond excited to say i’ve submitted my PhD thesis!!! A huge thanks to my supervisors AP Joanna Williams and Dr @RhysLivingstone for supporting me through all the ups and downs of my PhD journey
Image from Courteney Westlake (@CourteneyMW)
Image from Courteney Westlake (@CourteneyMW)
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First UK conference, first #NeuroBioUK, first @PDA_PhD lab poster, first place postdoc poster, and anniversary of my first year as a PD in Edinburgh🥇
Image from Dr. Rhys Livingstone (@RhysLivingstone)
Image from Dr. Rhys Livingstone (@RhysLivingstone)
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#fluorescencefriday DIV21 neurons 4x expansion Magenta- MAP2 Orange- SynGAP1 photo cred @RhysLivingstone
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This #FluorescenceFriday is our first attempt at imaging SynGAP in live hippocampal neurons during a cLTP protocol Thanks to @rhuganir and @yoichi_araki for their beautifully expressing (and dispersing!) SynGAP construct!
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When I joined @PDA_PhD’s lab I insisted it was on the condition he’d let me image spine dynamics (something I had yet the chance to do), safe to say the promise has been kept
Image from Dr. Rhys Livingstone (@RhysLivingstone)
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things are dire but sometimes you get a free jar of honey with your apology 🐝
Image from Dr. Rhys Livingstone (@RhysLivingstone)
Jason Shepherd (@JasonSynaptic) profile pictureJason Shepherd@JasonSynaptic13 Mar 2024
One of the most insidious problems in science…lack of antibody controls and verified antibodies. There really needs to be a standardized system where validated antibodies can be listed and antibodies that don’t work flagged. Would save countless $$ and time!
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fluorescence friday: after countless antibodies tested/tests (and failures)... SynGAP1 (pan, black) in DIV 21 cortical neurons. Magenta DAPI. perseverance credit to @RhysLivingstone
Image from Paul Donlin-Asp 🏳️‍🌈 (@PDA_PhD)
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First #FluorescenceFriday as a postdoc in the @PDA_PhD lab 10x expanded HEK cell nucleus (🔵) and newly synthesised proteins (🟣)
Image from Dr. Rhys Livingstone (@RhysLivingstone)
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Can confirm, 10x expanded nuclei are pretty neat
Andy Moore (@aaandmoore) profile pictureAndy Moore@aaandmoore6 Jan 2024
A 10× expanded nucleus stained with Hoechst to label DNA. At this expansion factor you can see gaps in the surface staining. Nuclear pores?
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Dr. Rhys Livingstone retweeted
Courteney Westlake (@CourteneyMW) profile picture
Courteney Westlake@CourteneyMW10 Nov 2023
First attempt at human iPSC-derived co-cultures: a funky beautiful mess of neurons 🔴, astrocytes 🟡 and newly synthesised proteins 🔵 #fluorescentfriday
Image from Courteney Westlake (@CourteneyMW)
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My nephews’ primary school class is currently learning about the body, so I couldn’t resist taking a trip to teach them about the most important organ (and make some pipe-cleaner neurons along the way) 🧠
Image from Dr. Rhys Livingstone (@RhysLivingstone)
Image from Dr. Rhys Livingstone (@RhysLivingstone)
Image from Dr. Rhys Livingstone (@RhysLivingstone)
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I’ve been putting off tweeting this until it feels real (visa accepted, flights booked and it still doesn’t..) but i’m incredibly honoured (and excited) to say that I have accepted a postdoctoral position in the lab of @PDA_PhD, in Edinburgh 🏴󠁧󠁢󠁳󠁣󠁴󠁿
Image from Dr. Rhys Livingstone (@RhysLivingstone)
Image from Dr. Rhys Livingstone (@RhysLivingstone)
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I leave behind 11 years at @otago and 7 years in my lab with these incredible people ☺️
Image from Dr. Rhys Livingstone (@RhysLivingstone)
Image from Dr. Rhys Livingstone (@RhysLivingstone)
Image from Dr. Rhys Livingstone (@RhysLivingstone)
Image from Dr. Rhys Livingstone (@RhysLivingstone)
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Dr. Rhys Livingstone retweeted
Hugo Chrost (@chrost_hugo) profile picture
Hugo Chrost
@chrost_hugo26 May 2023
Calcium transient in hippocampal neurons (and astrocytes) treated with KCl. Credits: @RhysLivingstone #neuroscience
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Now to induce a form of chemical LTP ⚡️⚡️
Dr. Rhys Livingstone (@RhysLivingstone) profile pictureDr. Rhys Livingstone@RhysLivingstone28 Jul 2022
I can now say i’ve officially joined the live-cell imaging club 🤩 calcium transients in hippocampal neurons (and astrocytes) treated with KCl
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Dr. Rhys Livingstone retweeted
Rosario in Paris ᥫ᭡ (@chayito09) profile picture
Rosario in Paris ᥫ᭡
@chayito0915 Mar 2023
Una belleza de video: transporte de calcio en neuronas del hipocampo (y astrocitos) tratadas con KCl. El calcio regula procesos celulares como la liberación de neurotransmisores, el acople excitación-contracción, etc. Vía: @RhysLivingstone
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Second milestone accomplished - learning live-cell imaging, building a drug perfusion system, and getting to watching these guys dance 🕺🏼
Dr. Rhys Livingstone (@RhysLivingstone) profile pictureDr. Rhys Livingstone@RhysLivingstone31 Jan 2023
First milestone of 2023 accomplished - growing ipsc-derived human gutamatergic and gabaergic neurons (GFP-expressing too!) @BrainXell_Inc
Image from Dr. Rhys Livingstone (@RhysLivingstone)
Image from Dr. Rhys Livingstone (@RhysLivingstone)
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Dr. Rhys Livingstone retweeted
Hugo Chrost (@chrost_hugo) profile picture
Hugo Chrost
@chrost_hugo6 Mar 2023
Super-resolved cell surface GluA1, GluA2, and GluA3 to start the week off. Credits: @RhysLivingstone #neuroscience #MedTwitter
Image from Hugo Chrost (@chrost_hugo)
Image from Hugo Chrost (@chrost_hugo)
Image from Hugo Chrost (@chrost_hugo)
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