@Saxena_Lab

The Saxena Lab at the University of Pittsburgh is an Electron Paramagnetic Resonance (EPR) spectroscopy group with interests in biophysics and methodology

 University of Pittsburgh
saxenalab.pitt.edu/
Joined May 2021
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Happy to see this new paper from the @Saxena_Lab published in Chemistry, A European Journal: Endogenous Cu(II) Labeling for Distance Measurements on Proteins by EPR dx.doi.org/10.1002/chem.2024…

Endogenous Cu(II) Labeling for Distance Measurements on Proteins by EPR

Quick and easy endogenous spin labeling: This work shows that hypotonic swelling is a reproducible and facile method to endogenously label proteins with Cu(II) protein spin label in E. coli cells....

chemistry-europe.onlinelibrary.wiley.com
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In our recent collaborative work with @Saxena_Lab , we explored the dynamic nature of P. aeruginosa CueR copper transcription factor, showing differences than its E.coli homologue - chemistry-europe.onlinelibra…

The Dynamic Plasticity of P. aeruginosa CueR Copper Transcription Factor upon Cofactor and DNA...

EPR spectroscopy was employed to investigate the behavior of P. aeruginosa CueR copper transcription factor, revealing its responsiveness to the promoter sequence and a distinct mechanism from its...

chemistry-europe.onlinelibrary.wiley.com
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Welcome to NEW IES committee to serve 2024-27! Marina Bennati, Sunil Saxena, John Morton, Mihee Lim and Janet Lovet. @Saxena_Lab @SpinLovett @songihanlab Huge thanks to ougoing IES Committee members for their selfless a. A healthy society is one that evolves - help us do!
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Very excited that Zikri Hasanbasri from @Saxena_Lab at Pitt has joined #fourseasonstotalspectroscopy @UCDChem as a new postdoc. Fantastic!
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Really exciting results from @Saxena_Lab demonstrating submicromolar pulse dipolar EPR with nitroxide labels using DQC. Impressive results!
Saxena Lab@Saxena_Lab28 Sep 2023
Alysia and Zikri’s paper on Q-band nitroxide double quantum coherence measurements is now published online in J. Phys. Chem. Lett.! In this work, we were able to achieve 100% modulation depths and 50 nM distance measurements in 12 hours. pubs.acs.org/doi/10.1021/acs…

Double Quantum Coherence ESR at Q‑Band Enhances the Sensitivity of Distance Measurements at...

Abstract. Recently, there have been remarkable improvements in pulsed ESR sensitivity, paving the way for broader applicability of ESR in the measurement o

pubs.acs.org
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Come join us @Pitt_Chemistry - we're hiring for two #facultychemjobs positions in #compchem and analytical chemistry, starting in Fall '24 Theory: cfopitt.taleo.net/careersect… Analytical: cfopitt.taleo.net/careersect…
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“Store-bought is fine”: Sensitivity considerations using shaped pulses for DEER measurements on Cu(II) labels #EPR ift.tt/H6ZdR4O
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Congratulations to Alysia Mandato (@AlysiaMandato) and Sebastian Atwood for receiving the @Bridge12Tech prize for best Student Talk at the Rocky Mountain Conference (@rockymtnconf ) in Denver this week. Well deserved guys! #EPR
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This has now been published in @J_A_C_S pubs.acs.org/doi/10.1021/jac…. If you have read the preprint discussed in the quoted🧵nothing much changed in the final📜. Fun project between Alan's group @StAndMedicine, Hassane @mmwave_EPR @PhysAstroStAnd, us here in @StAndrewsChem

Investigating Native Metal Ion Binding Sites in Mammalian Histidine-Rich Glycoprotein

Abstract. Mammalian histidine-rich glycoprotein (HRG) is a highly versatile and abundant blood plasma glycoprotein with a diverse range of ligands that is

pubs.acs.org
@belab.bsky.social@Bela_Bode13 Jan 2023
This project was sparked by a bar conversation with Alan Stewart from @StAndMedicine during a @StAndrewsBSRC Firbush retreat. Could we possibly use EPR spectroscopy to understand the metal ion binding of mammalian histidine-rich glycoprotein (HRG)? 1/
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#DYK that Mildren Cohn pioneered the use of nuclear magnetic resonance (NMR) to determine how enzymes & other proteins behave during chemical reactions in the body. Learn more at fal.cn/3wOlz #WomensHistoryMonth
Image of Mildren Cohn

ALT Image of Mildren Cohn

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Also featured on the Cover of Issue 1 is the work of Saxena and colleagues (@Saxena_Lab) on the measurement of protein dynamics from site directed Cu(II) labeling 👉chemistry-europe.onlinelibra…
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20 successful years of the @Saxena_Lab So glad to be a part of it and to be able to connect with my colleagues who are friends now. :)
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Hello from @rockymtnconf!
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Announcing IVEM seminar on May 26, 9 am New York time by Malte Dreschner (U of Konstanz) “In-cell EPR techniques for Studying Intrinsically disordered proteins (IDPs)”
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A great Passover gift. Our recent manuscript in collaboration with @Saxena_Lab is now online. We used RT CW-EPR to follow dynamical changes in CueR during transcription. #EPR onlinelibrary.wiley.com/doi/…

Allostery‐driven changes in dynamics regulate the activation of bacterial copper transcription...

Metalloregulators bind and respond to metal ions by regulating the transcription of metal homeostasis genes. Copper efflux regulator (CueR) is a copper-responsive metalloregulator that is found in...

onlinelibrary.wiley.com
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Working hard on this! More news to come soon… Can’t wait to see everyone in St Andrews for the RSC ESR conference in June @RSC_ESR #RSCESR2022 #EPR_ESR @univofstandrews @PhysAstroStAnd
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The IES is looking for candidates to deliver a Bruker-organized webinar. This is important to maintain Bruker support in IES. Please contact [email protected] if you are interested. It is a good opportunity and a nice exposure to your research.
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Abstracts are now being accepted for the 61st Rocky Mountain Conference on Magnetic Resonance taking place July 25-29, 2022 in Copper Mountain, Colorado. The conference will feature the 43rd International EPR Symposium and the Solid-state NMR Symposium. rockychem.com/conference/cal…
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Long awaited community paper on DEER/EPR is out! “Benchmark Test and Guidelines for DEER/PELDOR Experiments on Nitroxide-Labeled Biomolecules: pubs.acs.org/doi/full/10.102… @SchiemannLab @Mchaourablab @prisnerlab @Bela_Bode @enricabordignon @JeschkeLab
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We're hiring! We seek to fill the position of a tenure track Assistant Professor, effective Sep. 2022, pending budgetary approval. Candidates in the general areas of quantum materials, quantum sensing and quantum computing will be considered. #chemjobs cfopitt.taleo.net/careersect…
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