New Chemxriv (now with link) doi.org/10.26434/chemrxiv.14… “Photoblueing” artifacts have been noted with a range of fluorescent probes including cyanines (for example Cy5, Cy7, etc.). It turns out that the polymethine gets shorter! So Cy7s turn into Cy5s, which turn into Cy3s (1/3).

Here is our new chemxriv. shorturl.at/quxDU “Photobluing” artifacts have been noted with a range of fluorescent probes including cyanines (for example Cy5, Cy7, etc.). It turns out that the polymethine gets shorter! So Cy7s turn into Cy5s, which turn into Cy3s (1/3).