@StaphEric

Microbiologist, works on Staphylococcus aureus, quorum sensing, evolution and virulence. Views are my own. I don't always wear the flat cap.

Joined June 2013
  • Tweets1K
  • Following804
  • Followers412
11Media
Scientists present a soft #biorobotic heart to assess the feasibility and safety of new valve designs and #biomaterials to correct #TetralogyofFallot, offering a reliable model to inform reconstructive heart surgery. @ScienceTM scim.ag/7MM
2
16
1
67
29,887
Study in #zebrafish shows the #heartbeat's mechanical forces remodel the developing heart's #extracellularmatrix. Image & research by Alessandra Gentile & @MargaAlbu et al @StainierLab @mpi_hlr @GunawanLab @wwu_muenster in @Dev_journal. On bpod.org.uk/archive/2024/7/2…
2
8
471
Eric Pollitt retweeted
Development@Dev_journal4 Jul 2024
Llgl1 mediates timely epicardial emergence and establishment of an apical laminin sheath around the trabeculating cardiac ventricle Read this Research Article by Eric Pollitt @StaphEric, Emily Noël @noelresearchlab and colleagues @ScienceShef: journals.biologists.com/dev/…
Figure 1 A-J: Llgl1 promotes organised trabeculation and ventricular wall integrity. (A-J) Live light-sheet z-slices through the ventricle of Tg(myl7:LifeAct-GFP) transgenic wild-type and llgl1 mutant embryos visualising the myocardium at 120 hpf (A-E) and 80 hpf (F-J). Scale bars: 50 μm. Wild-type embryos have regular trabeculae emerging predominantly from the outer curvature of the ventricular wall at 120 hpf (A), whereas llgl1 mutant embryos exhibit disorganised trabeculae, ranging from wild-type-like (B), through to irregular trabecular CMs and multilayering of CMs (C-E). Some llgl1 mutants at 120 hpf exhibit apically extruding CMs (arrow, E). At 80 hpf in llgl1 mutants, CMs extrude apically from multiple locations in the ventricular wall (arrows G-J).

ALT Figure 1 A-J: Llgl1 promotes organised trabeculation and ventricular wall integrity. (A-J) Live light-sheet z-slices through the ventricle of Tg(myl7:LifeAct-GFP) transgenic wild-type and llgl1 mutant embryos visualising the myocardium at 120 hpf (A-E) and 80 hpf (F-J). Scale bars: 50 μm. Wild-type embryos have regular trabeculae emerging predominantly from the outer curvature of the ventricular wall at 120 hpf (A), whereas llgl1 mutant embryos exhibit disorganised trabeculae, ranging from wild-type-like (B), through to irregular trabecular CMs and multilayering of CMs (C-E). Some llgl1 mutants at 120 hpf exhibit apically extruding CMs (arrow, E). At 80 hpf in llgl1 mutants, CMs extrude apically from multiple locations in the ventricular wall (arrows G-J).

2
6
1,373
ICYMI
Darius Balciunas@Balciunas_Lab26 Mar 2024
1/8 Are you interested in knocking genes out in adult zebrafish? If so, you should read our new preprint! Our new ~ubiquitous ubbR:CreERT2 driver enables gene knockout in adult #zebrafish hearts with efficiency approaching 100%. Yes, we quantified it. biorxiv.org/content/10.1101/…

Highly efficient tamoxifen-inducible Cre recombination in embryonic, larval and adult zebrafish

We have generated transgenic lines containing zebrafish-optimized CreERT2 recombinase under the control of a recombinant ubbR promoter consisting of the zebrafish ubiquitin promoter supplemented with...

biorxiv.org
1
5
1,424
We hope that many of its features can be utilised to understand morphology beyond the walls of the developing heart - so take it for a whirl, and let us know how you get on.
1
6
391
The preprint describing the software, and how we used it to analyse zebrafish heart morphogenesis, are on bioRxiv tinyurl.com/mvjd45ze, while morphoHeart itself is available on GitHub github.com/jsanchez679/morph…
1
1
4
610
morphoHeart is live! @J_SanchezPosada developed software for 3D visualisation and morphometric analysis of the heart including negative space segmentation of cardiac ECM volume. All packaged up into a handy GUI. #morphometrics #zebrafish
3
21
1
72
8,710
A wee 2024 reminder that I'm looking for a job. Ideally it would be in the UK or Ireland (Scotland would be the dream). Please give me a heads-up if you have an opening coming up or know someone who has. You can check out my interests & CV at rosssmcinnes.github.io/ Cheers! 😃
Dr Ross McInnes@roscobacterium14 Nov 2023
My time at Birmingham will very sadly be ending in March. If you're looking for a dashingly handsome, well-spoken postdoc, that's not me, but I can do wet and dry lab ([functional]genomics, metagenomics, RNAseq) work in Gram-positives (especially Enterococcus) so keep me in mind.
1
23
27
9,324
A real paradigm shift from Astrid Hendriks and @N_van_sorge on how to understand adaptive immunity to S. aureus IgM (not IgG) to wall teichoic acids predicts protection vs mortality in bacteremia—IgM superior in promoting opsonophagocytosis, including as a monoclonal therapeutic
Nina van Sorge@N_van_sorge17 Jul 2023
Did you ever wonder about the role of IgM in host defense against Staphylococcus aureus? In this preprint, we identify opsonic IgM to be critical for human immunity to S. aureus. Potential implications for antibody-based therapies and vaccines. biorxiv.org/content/10.1101/…

Glycan-specific IgM is critical for human immunity to Staphylococcus aureus

Staphylococcus aureus is a major human pathogen but the immune factors that protect against it remain elusive. In particular, high opsonic IgG titers achieved in preclinical S. aureus animal immuni...

biorxiv.org
12
39
6,604
Eric Pollitt retweeted
70s Sci-Fi Art@70sscifi16 Jul 2023
Star Wars immunization promotional poster, 1979
A photo of C3PO and R2D2, with the caption "Parents of Earth, are your children fully immunized?"

ALT A photo of C3PO and R2D2, with the caption "Parents of Earth, are your children fully immunized?"

4
88
8
577
52,839
One more #AskZebrafish 🤔 from Slack: What are the best antibodies for labeling zebrafish vasculature? We are talking antibodies and not transgenics 🙏
3
1
9
4,685
Eric Pollitt retweeted
Tim Chico@Timchico13 Apr 2023
Fantastic work by Dr Ryan Snodgrass and many colleagues.
DMM Journal@DMM_Journal13 Apr 2023
Brand new article from @timchico & co from @sheffielduni on vascular abnormalities in #zebrafish model of haemorrhagic #telangiectasia that were prevented by synergistic inhibition of MEK & mTOR pathways, suggesting potential novel therapeutic approach 📰journals.biologists.com/dmm/…
2
13
1,905
The method used to prepare Mycobacterium tuberculosis cells affects the bacterial cell wall, changing infection outcomes. elifesciences.org/articles/8…
Blue, red and yellow epifluorescence microscopy image

ALT Blue, red and yellow epifluorescence microscopy image

1
8
3
28
11,763
A chemical regularly used to image cells can dramatically alter the way cellular compartments called condensates look under the microscope. elifesciences.org/articles/8…
Liquid-liquid phase separation of some proteins in yellow in a live mammalian cell (left) can look very different upon paraformaldehyde fixation (right). Protein after fixation are separated and look granular, rather than a smooth yellow background

ALT Liquid-liquid phase separation of some proteins in yellow in a live mammalian cell (left) can look very different upon paraformaldehyde fixation (right). Protein after fixation are separated and look granular, rather than a smooth yellow background

1
18
95
32,252
This is all you need to read about the Group A Strep outbreak triggering sepsis in some children. An expert round-up from @SMC_London. Important advice and reassurance: sciencemediacentre.org/exper…
32
75
9
176
Important paper: Global mortality associated with 33 bacterial pathogens in 2019: a systematic analysis for the Global Burden of Disease Study 2019 thelancet.com/journals/lance…
1
84
3
217
ok sci-twitter - does anyone know of a good (i.e. affordable) web resource for storing cell imaging data where we're talking in the 10-100 terabytes range? have people figured out how to comply with the upcoming NIH data sharing mandates?
34
46
7
170
Eric Pollitt retweeted
Ruth Massey@ProfRuthMassey8 Sep 2022
Would anyone like to come help me set up my new lab in Cork? I’ll have several position (RA, PhD and postdoc) ready to advertise soon, plus plenty of funding opportunities we can apply for together. Beautiful place to live and work! @SEFSUCC @UCCMedHealth @UCC @Pharmabiotic
19
115
4
203
Eric Pollitt retweeted
Jacob Corn@jcornlab5 Aug 2022
Today in @natcomms we report #RecursiveEditing: a way to drive high levels of #CRISPR #HDR without manipulating the cellular factors or state. Project co-led by Masters' student Lukas Möller and postdoc Eric Aird. nature.com/articles/s41467-0… 🧵

Recursive Editing improves homology-directed repair through retargeting of undesired outcomes

Nature Communications - CRISPR-Cas induced HDR methods tend to have a low efficiency. Here the authors report an HDR improvement strategy, Recursive Editing, that selectively retargets undesired...

nature.com
9
53
6
196
See hype around #monkeypox is gathering pace: 1. This outbreak is unusual both in scale and in potential common transmission route. 2. BUT This is very unlikely to be covid-like because:
6
22
2
101
Load more