@_AJSethi

RNA: splicing, modification + transcription (he/him)

 Australia
Joined June 2014
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SWARM (Single-molecule Workflow for Analysing RNA Modifications) detects pseudouridine, m6A, and m5C on individual molecules from direct RNA nanopore signals (in both chemistries RNA002 and RNA004) github.com/comprna/SWARM see more on poster P2-396 at #rna2025 #RNA25 #RNAmods

GitHub - comprna/SWARM: Single-molecule Workflow for Analysing RNA Modifications

Single-molecule Workflow for Analysing RNA Modifications - comprna/SWARM

github.com
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We describe the temporal organisation of central pre-mRNA processing events, including splicing, m6A modification and 3' end formation, during mRNA synthesis. Check out the preprint here!
Eduardo Eyras@EduEyras29 Apr 2025
"Single-molecule multimodal timing of in vivo mRNA synthesis" a collaboration with Rippei Hayashi's lab, work led by AJ Sethi @_AJSethi #RNA #premRNA #splicing #RNAprocessing #m6A #polyadenylation biorxiv.org/content/10.1101/…

Single-molecule multimodal timing of in vivo mRNA synthesis

mRNA synthesis requires extensive pre-mRNA maturation, the organisation of which remains unclear. Here, we directly sequence pre-mRNA without metabolic labelling or amplification to resolve transcr...

biorxiv.org
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dFORCE is directly applicable to mammalian tissue samples, and produces results consistent with cell culture, unlocking analysis of mRNA synthesis kinetics in vivo:
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Thanks to all co-authors, without whom this work would be impossible. @GuarnacciMarco @starstuffkarthi @pol2rna @TominAustralia @RippeiH and others.
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it gets me fr
Linus ✦ Ekenstam
@LinusEkenstam8 Nov 2024
Ask ChatGPT “based on what you know about me. draw a picture of what you think my current life looks like” past your responses below. thanks again @mreflow & @danshipper
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R2Dtool github.com/comprna/R2Dtool for handling, integrating, and viualising isoform-resolved RNA feature data - led by @_AJSethi #bioinformatics #RNA #isoforms academic.oup.com/bioinformat…

GitHub - comprna/R2Dtool: Analyse RNA feature distributions.

Analyse RNA feature distributions. Contribute to comprna/R2Dtool development by creating an account on GitHub.

github.com
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AJ Sethi retweeted
Cell@CellCellPress5 Jun 2024
Now online! Capturing totipotency in human cells through spliceosomal repression dlvr.it/T7tnPv
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It's finally published. This one took a long time to get through reviewers: Prediction of m6A and m5C at single-molecule resolution reveals a transcriptome-wide co-occurrence of RNA modifications link.springer.com/article/10…

Prediction of m6A and m5C at single-molecule resolution reveals a transcriptome-wide co-occurrence...

Nature Communications - The epitranscriptome holds many unexplored RNA functions, but detecting multiple modifications from one sample remains challenging. Here, authors devise a strategy combining...

link.springer.com
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Jonas Salk, inventor of polio vaccine: "There is no patent. Could you patent the sun?" Frederick Banting, inventor of insulin: "Insulin does not belong to me. It belongs to the world." Moderna CEO Stéphane Bancel: 400% price hike on COVID vaccine is "consistent with the value."
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Kicking off a week of incredible Australian bioinformatics research with the COMBINE 2022 Symposium! @combine_au @abacbs #COMBINE22
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🪐 Introducing Galactica. A large language model for science. Can summarize academic literature, solve math problems, generate Wiki articles, write scientific code, annotate molecules and proteins, and more. Explore and get weights: galactica.org
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Did you know #PhD students in Australian #academia, people with a professional level of education, live ~$80 a week below the poverty line? PhD students account for about 33% of #academic output. Maybe it's time we unionised.
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Simultaneous detection of m6A and m5C on individual RNA molecules using CHEUI (CH3 Estimation Using Ionic current). Thanks to everyone who helped!! @_AJSethi @EduEyras @GuarnacciMarco @tominaustralia ...etc #RNAmodifications #RNA #DeepLearning biorxiv.org/content/10.1101/…

Identification of m6A and m5C RNA modifications at single-molecule resolution from Nanopore...

The expanding field of epitranscriptomics might rival the epigenome in the diversity of the biological processes impacted. However, the identification of modifications in individual RNA molecules...

biorxiv.org
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A meeting not to be missed! Join us for the inaugural A-RNA meeting 15-18th May, amazing speaker line up and most of all, an oppurtunity be amongst the cool gang who love the other nucleic acid @ARNAconference #RNA
Weatheritt Lab@Neuro_RNA21 Feb 2022
Australasian RNA Biology and Biotechnology Conference (A-RNA - a-rna.org) conference. 15-18th May! Melissa Moore and Howard Chang giving Keynote lectures! 2/3 of time for submitted abstracts! Register now and join us in Thredbo!
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Submitting my work to nature… #academictwitter #phdchat #lornegenome ⁦@GenomeConf⁩
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Can't belive in-person conferences are back!!! Road trip to @GenomeConf with @renzobalb and @_AJSethi!!
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genetic blockchain
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And that's a wrap for #COMBINE21! Thanks to my incredible co-chair @sachintha_wije & our committee, our speakers, career panelists, Prof. @AliciaOshlack, and finally, all our attendees. Proudly supported by @PawseyCentre @IntersectAust @MelBioInf @SA_genomics. Onto #ABACBS2021!!
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