It’s often said that there’s no biomarker for ME/CFS, Long Covid, Post Vaccine Syndrome, PSSD, Lyme Disease, etc. I strongly disagree. However, it helps to have the right data and tools to visualise it. To help people explore the Born Free disease model and better understand the connections between the minerals deficiencies, metal toxicities and disease model processes, I've added 10 random example pairs of Oligoscan (corrected, skin minerals + toxic metals) + Theriome Aristotle Plus data (296 metabolites) to the Metabolic Pathway Overlay tool. bornfree.life/learn/metaboli… Some common patterns to observe across these patient profiles: 1) Elevated toxic metals, with mineral and electrolyte deficiencies, relating to pathway alterations. 2) A reduced total NADP/NADPH pool size and impaired conversion of NADP to NADPH (low NADPH), with broad effects. The reported exception (#4) is where aluminium is not elevated. 3) Constraints on glycolysis and glycogen synthesis, gluconeogenesis. 4) Altered pyruvate and/or lactate levels, ratio. 5) Constraints on the pentose phosphate pathway and downstream PRPP synthesis. 6) Impairments in purine and pyrimidine metabolism, Cell Danger Response. 7) Oxidative stress. 8) TCA cycle alterations and constraints. 9) Issues with NAD+ synthesis and redox. 10) Reduced carnitine synthesis and associated carnitine-shuttle constraints. 11) Elevated glutamate relative to low GABA, except where severe induced malnutrition constrains glutamate synthesis. 12) Issues with glutathione metabolism. 13) Issues involving magnesium and Mg-ATP. 14) Urea-cycle and nitric oxide synthase impairments, with potential effects on vascular tone. 15) Dysregulated neurotransmitter synthesis and degradation. 16) Impaired conversion of choline to betaine. 17) Impaired methylation and associated SAMe, glycine, and cysteine metabolism. 18) Impaired creatine synthesis. Importantly, you can see coherence between the tests and inferred causality for pathway alterations. When you consider these metabolic alterations in specific cells / tissues - brain, liver, kidneys, thyroid, immune, etc., it becomes much easier to understand how mitochondrial dysfunction creates systemic impacts and a progressive cascade.