Yi (Eason) SHEN 沈溢 (@easonyshen) header
Yi (Eason) SHEN 沈溢 (@easonyshen) profile picture

Yi (Eason) SHEN 沈溢

@easonyshen

Scientist & Protein Engineer | Husband & Dad

 Boston, MA
scholar.google.ca/citations?user=kXStPmUAAAAJ&hl=en
Joined July 2012
  • Tweets375
  • Following665
  • Followers594
18Media
Photo by Yi (Eason) SHEN 沈溢 (@easonyshen)Photo by Yi (Eason) SHEN 沈溢 (@easonyshen)Photo by Yi (Eason) SHEN 沈溢 (@easonyshen)Photo by Yi (Eason) SHEN 沈溢 (@easonyshen)Photo by Yi (Eason) SHEN 沈溢 (@easonyshen)Photo by Yi (Eason) SHEN 沈溢 (@easonyshen)Photo by Yi (Eason) SHEN 沈溢 (@easonyshen)Photo by Yi (Eason) SHEN 沈溢 (@easonyshen)Photo by Yi (Eason) SHEN 沈溢 (@easonyshen)Photo by Yi (Eason) SHEN 沈溢 (@easonyshen)
Yi (Eason) SHEN 沈溢 retweeted
Abhi Aggarwal, MD (@abhi_aggarwal1) profile picture
Abhi Aggarwal, MD@abhi_aggarwal14 Apr 2024
Thrilled to share #TGECO1, a new blue-shifted Ca2+ indicator for imaging neural activity, freshly published in Neurophotonics! Large 2PX, great for multiplexing! doi.org/10.1117/1.NPh.11.2.0… @Campbell_FPs @easonyshen @PodgorskiLab @AllenInstitute @HHMIJanelia @UCalgary
3
22
107
7,465
Yi (Eason) SHEN 沈溢 retweeted
Ahmed Abdelfattah (@Abdelfattah_Lab) profile picture
Ahmed Abdelfattah@Abdelfattah_Lab20 Dec 2023
We are recruiting for an open Postdoc position. Interested in developing molecular tools? Our research is at the intersection of neuroscience, biochemistry, and biophysics. Please spread the word. Reach out via email if interested! @BrownUNeuro @CarneyInstitute
7
16
31
8,863
Yi (Eason) SHEN 沈溢 retweeted
Joachim Goedhart (@joachimgoedhart) profile picture
Joachim Goedhart@joachimgoedhart28 Oct 2023
Red fluorescent proteins engineered from green fluorescent proteins pnas.org/doi/abs/10.1073/pna…
Image from Joachim Goedhart (@joachimgoedhart)
2
57
1
238
33,836
Yi (Eason) SHEN 沈溢 retweeted
Abhi Aggarwal, MD (@abhi_aggarwal1) profile picture
Abhi Aggarwal, MD@abhi_aggarwal118 Oct 2023
Thrilled to share a new teal Ca2+ indicator! #TGECO is a blue-shifted biosensor with high SNR & enhanced 2P absorption, great for #multiplexing in vivo!🧵 See preprint below⬇️ biorxiv.org/content/10.1101/… @Campbell_FPs @easonyshen @PodgorskiLab @AllenInstitute @HHMIJanelia @UCalgary
T-GECO1 indicator structure

ALT T-GECO1 indicator structure

5
26
2
76
14,595
Yi (Eason) SHEN 沈溢 retweeted
Joachim Goedhart (@joachimgoedhart) profile picture
Joachim Goedhart@joachimgoedhart15 Sep 2023
Photoswitchable binders enable temporal dissection of endogenous protein function biorxiv.org/content/10.1101/…
Image from Joachim Goedhart (@joachimgoedhart)
22
1
131
15,929
Yi (Eason) SHEN 沈溢 retweeted
Helen Farrants (@HelenFarrants) profile picture
Helen Farrants@HelenFarrants20 Jul 2023
Our #chemigenetic #fluorescent calcium indicator WHaloCaMP is now out on #bioRxiv : biorxiv.org/content/10.1101/… WHaloCaMP can be used to report on neuronal activity in flies, zebrafish and mice. WHaloCaMP is great for #multiplexedimaging. A 🧵:
7
62
8
175
38,857
Introducing LSSmApple, a long Stokes shift red fluorescent protein, enabling the construction of 2nd gen dual-FP Matryoshka sensors for high throughput quantitative analysis with minimal spectral bleed-thru. biorxiv.org/content/10.1101/…
A monochromatically excitable green-red dual-fluorophore fusion incorporating a new large Stokes...

A monochromatically excitable green-red dual-fluorophore fusion incorporating a new large Stokes...

Genetically encoded sensors enable quantitative imaging of analytes in live cells. State-of-the-art sensors are commonly constructed by combining ligand-binding domains with one or more sensitized...

biorxiv.org
2
2
21
2,090
It's been a great pleasure to work with @jejike1 @MayuriSadoine Tom Kleist @healthy_crop & others on this project! Stay tuned for more follow-ups. LSSmApple plasmid is available on @Addgene addgene.org/191259/
pBAD-LSSmApple

pBAD-LSSmApple

Plasmid pBAD-LSSmApple from Dr. Robert Campbell's lab contains the insert LSSmApple and is published in Biochemistry. 2024 Jan 2;63(1):171-180. doi: 10.1021/acs.biochem.3c00451. Epub 2023 Dec 19....

addgene.org
1
3
852
Yi (Eason) SHEN 沈溢 retweeted
Andrew York (@AndrewGYork) profile picture
Andrew York@AndrewGYork12 Jul 2023
We discovered a surprisingly easy way to control the brightness of fluorescent proteins via magnetic fields: andrewgyork.github.io/gfp_ma… This raises fascinating scientific questions about the mechanism, and tantalizing technological opportunities for bioengineering.
44
303
74
1,301
230,733
Yi (Eason) SHEN 沈溢 retweeted
Prof. Michael Lin (@MichaelLinLab) profile picture
Prof. Michael Lin@MichaelLinLab6 Jul 2023
Today in @naturemethods: our new positively tuned voltage indicator, ASAP4e, for extended electrical recordings in the brain. ASAP4e achieves greater responsivity (>200% per 100mV vs 50% for other fluorescent voltage sensors) and better photostability. nature.com/articles/s41592-0…
A positively tuned voltage indicator for extended electrical recordings in the brain

A positively tuned voltage indicator for extended electrical recordings in the brain

Nature Methods - The ASAP4 family of genetically encoded voltage indicators allows recording of action potentials and subthreshold activity with either one- or two-photon microscopy over extended...

nature.com
7
57
8
210
38,469
Yi (Eason) SHEN 沈溢 retweeted
Abhi Aggarwal, MD (@abhi_aggarwal1) profile picture
Abhi Aggarwal, MD@abhi_aggarwal14 May 2023
iGluSnFR3 is published! Check it out⬇️ nature.com/articles/s41592-0… We made a new glutamate indicator with improved sensitivity, kinetics & localization for imaging synaptic transmission. This is #iGluSnFR3 in action detecting minis w/ TTX. @PodgorskiLab @HHMIJanelia @AllenInstitute
27
245
47
1,009
131,683
Yi (Eason) SHEN 沈溢 retweeted
Yusuke Nasu (@NasuLab_IBC) profile picture
Yusuke Nasu
@NasuLab_IBC29 Dec 2022
The latest versions of genetically encoded L-lactate biosensors, LACCO series, are out in bioRxiv: a second-generation green fluorescent extracellular biosensor eLACCO2.1 and a red fluorescent intracellular biosensor R-iLACCO1. #lactate #biosensor biorxiv.org/content/10.1101/…
Improved genetically encoded fluorescent biosensors for monitoring of intra- and extracellular...

Improved genetically encoded fluorescent biosensors for monitoring of intra- and extracellular...

l-Lactate is increasingly appreciated as a key metabolite and signaling molecule in mammals. To enable investigations of both the inter- and intra-cellular dynamics of l-Lactate, we develop a...

biorxiv.org
4
11
44
7,973
Yi (Eason) SHEN 沈溢 retweeted
Ben Campbell (@lysergicdesigns) profile picture
Ben Campbell@lysergicdesigns7 Nov 2022
Our article reporting hyperfolder YFP (#hfYFP) and other fixative-resistant fluorescent proteins based on #mGreenLantern is now available at @naturemethods. hfYFP enhances electron- and expansion-microscopies, protein purification, and so much more! 🧵nature.com/articles/s41592-0…
Chemically stable fluorescent proteins for advanced microscopy

Chemically stable fluorescent proteins for advanced microscopy

Nature Methods - The engineered hyperfolder YFP (hfYFP) and variants offer unprecedented chemical and thermal stability, making them versatile probes for microscopy as well as for challenging...

nature.com
12
122
24
500
Yi (Eason) SHEN 沈溢 retweeted
PLOS Biology (@PLOSBiology) profile picture
PLOS Biology@PLOSBiology7 Sep 2022
#Potassium ions are an essential electrolyte in all biological systems. @easonyshen @Campbell_FPs &co describe high-performance genetically encoded K+ sensors to enable in vivo measurement of [K+] across multiple model organisms #PLOSBiology plos.io/3BkdEa3
Image from PLOS Biology (@PLOSBiology)
9
14
Yi (Eason) SHEN 沈溢 retweeted
Yusuke Nasu (@NasuLab_IBC) profile picture
Yusuke Nasu
@NasuLab_IBC1 Sep 2022
Are you interested in a red fluorescent genetically encoded biosensor for extracellular L-lactate? Please check out our preprint on R-eLACCO2! biorxiv.org/content/10.1101/… Thank you so much, everyone contributing to this project! @Campbell_FPs
A red fluorescent genetically encoded biosensor for extracellular L-lactate

A red fluorescent genetically encoded biosensor for extracellular L-lactate

L-Lactate, traditionally recognized as a waste product of metabolism, is now appreciated as a key intercellular energy currency in mammals. To enable investigations of intercellular shuttling of...

biorxiv.org
2
11
48
Yi (Eason) SHEN 沈溢 retweeted
Alexey Chizhik (@ChizhikAlexey) profile picture
Alexey Chizhik@ChizhikAlexey28 Aug 2022
Inspired by a fantastic sculpture recently made by @mtyka. #SciArt
8
72
4
548
Clearing the lab fridges today and found these gems! Happy #fluorescencefriday
Image from Yi (Eason) SHEN 沈溢 (@easonyshen)
Image from Yi (Eason) SHEN 沈溢 (@easonyshen)
14
Yi (Eason) SHEN 沈溢 retweeted
Yong Qian (@YongQian6) profile picture
Yong Qian@YongQian624 Aug 2022
How to do “multicolor imaging” with a “single color channel” on an ordinary fluorescent microscope? Here we show that temporally multiplexed imaging (TMI), a new principle of imaging, can do this magic.
Image from Yong Qian (@YongQian6)
7
24
2
110
Yi (Eason) SHEN 沈溢 retweeted
Yulong Li Lab (@yulonglilab) profile picture
Yulong Li Lab@yulonglilab29 Jun 2022
Dopamine signals in RED! New rGRAB-DA sensors provide much higher in vivo sensitivity and SNR, and enable multiplex imaging with green sensors. #GRAB_sensors
Image from Yulong Li Lab (@yulonglilab)
2
59
1
352
Yi (Eason) SHEN 沈溢 retweeted
Yulong Li Lab (@yulonglilab) profile picture
Yulong Li Lab@yulonglilab28 Jun 2022
Greatly improved green GRAB-DA sensors enable detection of cortical dopamine dynamics in vivo!
Image from Yulong Li Lab (@yulonglilab)
7
84
1
421
Load more