Excited to introduce CRISPR-All! – a flexible, high-throughput framework that combines virtually any perturbation modality (CRISPRko, CRISPRi, CRISPRa, shRNA, knock-in, base/prime editing, etc.) in the same cell, at scale. Paired with single-cell RNA-seq @10xGenomics and rich phenotypic readouts, it unlocks massively combinatorial genetic screens and new cell states previously out of reach. Outstanding work led by @Roth_Lab @AustinMHartman biorxiv.org/content/10.1101/…

Happy to share our new work describing DESynR genes! The evolution of new human protein-coding genes relies on repurposing protein domains from ancestral genes, rather than de novo sequence creation. New genes evolved from old parts, and while we have methods that enable high-throughput generation and screening of base-pair level sequence evolution (mutagenesis, directed evolution, CRISPR, etc), we lack similar scalable technologies for domain-level evolution. We developed a high-throughput combinatorial molecular assembly method to generate thousands of barcoded novel genes from the domain building blocks of natural genes, termed DESynR (Domain Engineered via Synthesis and Recombination) genes.