Excited that a talented Ph.D. student Mohammed Aljohani @MDJEditing from my lab is presenting his work on a genome-wide C. elegans CRISPR library for DNA and RNP-based CRISPR editing for the first time today at the CSHL CRISPR meeting.

Congrats Julie & team! Thanks for the huge effort to make a reference. Oh, and @AGCharlesworth graphics really are beautiful. I am putting this panel up in the lab wall for reference.

SGB lab members at the European Worm Meeting 2022!

6/7 The optimized sequence is annotated in the app or in a GenBank file that can be opened in gene editors (for example @ApEplasmid)

5/7 We frequently synthesize full genes but find that some sequences cause trouble. The app checks for problematic features

4/7 And remove common restriction enzyme sites to facilitate downstream cloning

3/7 You can add endogenous or synthetic introns and UTRs (5' and 3')

2/7 The app can also remove or annotate piRNA homology (to reduce germline silencing)

1/7 Try our new app for optimizing transgenes for C. elegans expression (wormbuilder.org/transgenebui…). The app uses algorithms from collaborators Henrik Bringmann (TU Dresden) and Dan Dickinson (UT Austin) - or codons from highly expressed ubiquitous genes.

4/5 We integrated a > 5.4 Mb simple plasmid array and @FaisalMAlkhaldi assembled it from long-read Oxford Nanopore sequencing. Tandem arrays and truncated plasmids as first shown by Stinchcomb in 1985. First baby steps in trying to assemble larger synthetic genomic regions.

2/5 MosTI allows easier and more efficient insertion of single-copy and extra-chromosomal arrays into safe harbor sites on three chromosomes. We generated a large set of reagents that are available at CGC, Addgene, and Twist. See poster for reagent overview.