Biochemist. Transitioning from studying protein conformation in a tube @JNCASR to cells @TheCrick with @balchin_david using mass spectrometry.
Gas phase
Joined March 2014
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Is there any report in literature on passivating beads using a Lys/Arg-free protein for pull-down or IP-MS? #TeamMassSpec
@ItsBiniR
@UCDProteomics
@ProteomicsNews
Starting with 2 ug of protein for S-trap digestion, I end up with 15 ug of tryptic peptides as reported by Thermo BCA colorimetric peptide assay. Where am I going wrong? #TeamMassSpec @ItsBiniR @UCDProteomics @ProteomicsNews
santosh retweeted
“I'd also like to acknowledge my students (…) they've gone on to do many great things.
I'm particularly proud of the fact that one of my students fired Sam Altman.“
😳🫡
santosh retweeted
New from our team @TheCrick, led by @GrantPellowe. Eukaryotes are very good at folding multidomain proteins. Here we give some insight into how the human ribosome helps with this, by tuning how nascent domains fold and dock. 1/n biorxiv.org/content/10.1101/…
santosh retweeted
Our #FragPipe-Analyst manuscript is out! pubs.acs.org/doi/10.1021/acs…. Load FragPipe's TMT/ LFQ/DIA quant tables (peptide or protein quant) and get QC, Limma, PCA, volcano plots, heatmaps, do GO/pathway enrichment analysis, all with just a few clicks! Tutorials:fragpipe-analyst-doc.nesvila…
santosh retweeted
I wish journals would require methods papers be reproducible by at least 3 labs before it can be published . Think of all the time and resources this would save !
santosh retweeted
Eric Schmidt says in the next year, AI models will unite three key pillars: very large context windows, agents and text-to-action, and no-one understands what the impact will be but it will involve everyone having a fleet of AI agents at their command
santosh retweeted
New analysis from the @royalsociety shows just how high UK visa costs for researchers are compared to upfront costs for similar visa routes in 13 other countries.
Here, we take a look at how the UK compares to countries in the G7 included in the analysis 🔍⤵️ 1/5
ALT Bar chart shows the upfront costs for similar visa routes for researchers in the UK, US, France, Italy, Germany and Japan. UK costs are multiple times higher than other countries and the biggest factor for this is the Immigration Health Surcharge (IHS).
santosh retweeted
Proud to share our work on molecular chaperones, previously posted as @biorxivpreprint, now out in @MolecularCell
@balchin_david @SarahMaz76 @funtoshs @GrantPellowe @SkehelJM @TheCrick
sciencedirect.com/science/ar…
santosh retweeted
Join us for a PhD! I am recruiting a student to explore uncharted space in protein biogenesis, together with an exceptional team in a fantastic research environment @TheCrick. Fully funded and open to any nationality crick.ac.uk/careers-study/va…
santosh retweeted
Words of advice: Teaching macromolecular crystallography
@ECA_social @ACAxtal @Whatsinacrystal @britcryst @CNC_Crystals @buildmodels @PDBeurope @ProteinMagnus @ASBMB @IUCr @BiochemSoc @BiophysicalSoc @crystallo_world @ccp4_mx
Plz RT for greater outreach
#XRAY #crystallography
I am delighted to present this Words of Advice article on Teaching macromolecular crystallography that talks about the difficulties inherent in teaching & learning of this subject & possible approaches to overcome them for a complete appreciation.
febs.onlinelibrary.wiley.com…
santosh retweeted
Registration is open for the 33rd annual meeting of the UK Chaperone Club on 17 April 2023! Sessions will cover topics including the structure and folding of molecular chaperones and cellular stress responses. @Proteintech | @NEBiolabs
crick.ac.uk/whats-on/uk-chap…
santosh retweeted
3yr #postdocposition in Cambridge @AstraZeneca, split between their shiny new DISC & our lab @BabrahamInst. Help explore how cellular stresses shape #PROTAC efficacy, & vice-versa. Fundamental/translational relevance aplenty! Oh, some cool #proteomics too. careers.astrazeneca.com/job/… – at Babraham, England
santosh retweeted
I’m very proud to share the first paper from my lab @TheCrick, a wonderful collaboration with @jrengen. Made possible by Aleksandra Pajak and @tmonsterphd, with contributions from @funtoshs and @BetkaRoe. A summary🧵: 1/11 biorxiv.org/content/10.1101/…
santosh retweeted
NH3 channeling enzymes typically form tight complexes, but the M jannaschii GMP synthetase subunits interact transiently. Balaram and colleagues @jncasr now present a mechanistic model explaining the various steps in this fascinating catalytic cycle.
pubs.acs.org/doi/full/10.102…
Consider designing a two-component (A, B) enzyme system where A and B catalyze distinct reactions, A binds the cognate substrates and activates B, the product of B in-turn tunnels intermolecularly to the active site of A, reacts with the product of A, generating the final product
Irrespective of whether the design is tractable or not, thanks to evolution, such an enzyme complex exists. Here we dissect the functioning of an archaeal GMP synthetase using #Biochemistry #xraycrystallography and #XLMS. More on it here biorxiv.org/content/10.1101/…
My thanks and gratitude to all the co-authors in @jncasr namely Sanjeev Kumar (co-first author), @BellurAsutosh @SatyaDev2064 and my advisor Prof. Hemalatha Balaram.